Effect of additives and fed-batch culture strategies on the production of glutathione by recombinant Escherichia coli
Effect of additives and fed-batch culture strategies on the production of glutathione by recombinant Escherichia coli
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DOI：
10.1016/s0032-9592(98)00038-7
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发表时间：
1998-09-01
影响因子：
4.4
通讯作者：
Koo, YM
中科院分区：
文献类型：
--
作者：
Li, Y;Chen, J;Koo, YM
研究了酵母提取物、前体氨基酸和ATP对重组大肠杆菌生产谷胱甘肽（GSH）的影响。前体氨基酸（谷氨酸、半胱氨酸和甘氨酸）和ATP的加入促进了细胞内谷胱甘肽的积累。当发酵开始时和发酵12 h后分别添加2.0 g/l ATP和9 mmol/l前体氨基酸时，细胞浓度和细胞内GSH含量分别比未添加的培养高24%和1.4倍。当初始葡萄糖浓度大于20 g/l时，观察到生长抑制。在对分批培养过程进行分析的基础上，采用三种简单的补料策略，在罐式发酵罐中进行了不同补料-分批培养。指数饲养策略提高了细胞密度、生产力和生物量产量。在无纯氧条件下，25 h培养液中细胞密度和总GSH量分别达到80 g(DCW)/l和880 mg/l。1998爱思唯尔科学有限公司版权所有。
The effect of additives including yeast extract, precursor amino acids and ATP on the production of glutathione (GSH) by recombinant E. coli was investigated. The addition of precursor amino acids (glutamate, cysteine and glycine) and ATP improved intracellular GSH accumulation. When 2.0 g/l ATP and 9 mmol/l precursor amino acids were added at the beginning and after 12 h of fermentation, respectively, cell concentration and intracellular GSH content was 24% and 1.4 times higher than that of culture without additions. Growth inhibition was observed while the initial glucose concentration was above 20 g/l. Based on the analysis of the batch culture process, different fed-batch cultures in jar fermenters were carried out in which three kind of simple feeding strategies were applied. An exponential feeding strategy increased cell density, productivity and biomass yield. Using this strategy, cell density and total GSH quantity in broth reached 80 g(DCW)/l and 880 mg/l at 25 h without pure oxygen, respectively. (C) 1998 Elsevier Science Ltd. All rights reserved.