Physiological Activity of Campylobacter jejuni Far below the Minimal Growth Temperature
Physiological Activity of Campylobacter jejuni Far below the Minimal Growth Temperature
复制标题
远低于最低生长温度的空肠弯曲菌的生理活性
DOI：
10.1128/aem.64.10.3917-3922.1998
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发表时间：
1998
影响因子：
4.4
通讯作者：
T. Abee
中科院分区：
文献类型：
--
作者：
W. Hazeleger;J. Wouters;F. Rombouts;T. Abee
摘要通过测定人体内空肠弯曲菌的生理活性，研究了该菌在环境温度下的行为。菌株104和ATCC 33560的最低生长温度分别为32℃和31℃。两个菌株的生长速率都在最高生长温度附近和最低生长温度附近的几度内突然从最大值下降到零。这可能表明，关键酶(S)或调节化合物(S)的结构依赖于温度的转变决定了最低生长温度。在低至4°C的温度下，观察到了氧消耗、过氧化氢酶活性、ATP合成和蛋白质合成，表明重要的细胞过程仍然在发挥作用。PCR分析表明，在许多细菌中起低温适应作用的冷休克蛋白基因并不存在。空肠。在所有温度下都能观察到趋化性和趋气性，这表明病原菌能够在环境温度下移动到有利的地方，这可能对空肠弯曲菌在环境中的生存具有重要意义。
ABSTRACT The behavior of Campylobacter jejuni at environmental temperatures was examined by determining the physiological activities of this human pathogen. The minimal growth temperatures were found to be 32 and 31°C for strains 104 and ATCC 33560, respectively. Both strains exhibited a sudden decrease in growth rate from the maximum to zero within a few degrees not only near the maximal growth temperature but also near the minimal growth temperature. This could be an indication that a temperature-dependent transition in the structure of a key enzyme(s) or regulatory compound(s) determines the minimal growth temperature. Oxygen consumption, catalase activity, ATP generation, and protein synthesis were observed at temperatures as low as 4°C, indicating that vital cellular processes were still functioning. PCR analysis showed that cold shock protein genes, which play a role in low-temperature adaptation in many bacteria, are not present inC. jejuni. The fact that chemotaxis and aerotaxis could be observed at all temperatures shows that the pathogen is able to move to favorable places at environmental temperatures, which may have significant implications for the survival of C. jejuni in the environment.