Study of Cu chemical state inside single neurons from Parkinson's disease and control substantia nigra using the micro-XANES technique
Study of Cu chemical state inside single neurons from Parkinson's disease and control substantia nigra using the micro-XANES technique
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DOI：
10.1016/j.jtemb.2008.03.006
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发表时间：
2008-01-01
影响因子：
3.5
通讯作者：
Lankosz, Marek
中科院分区：
文献类型：
--
作者：
Chwiej, Joanna;Adamek, Dariusz;Lankosz, Marek
帕金森病（PD）被称为特发性障碍。这意味着它的原因还没有找到。然而，有一些过程，例如氧化应激。在这种神经退行性疾病中，蛋白质聚集和线粒体功能障碍被怀疑会导致黑质 (SN) 神经元萎缩和死亡。 Cu 是一种微量元素，其在 PD 发病机制中的作用受到广泛讨论。对 PD SN 和对照病例的单个神经细胞内 Cu 氧化态的研究可能会为该元素在 PD 中的作用提供一些新的线索。使用 X 射线吸收近边结构 (XANES) 光谱研究了 Cu 化学状态的差异。应用最小二乘拟合方法来分析 XANES 光谱。在能量尺度上白线多重散射和前边缘峰最大值的位置的比较没有揭示PD和对照样品之间Cu化学态存在差异。然而，人们发现SN神经元内的大部分Cu存在于四面体环境中，并且很可能是Cu(II)。 (C) 2008 爱思唯尔有限公司。版权所有。
Parkinson's disease (PD) is referred to as Idiopathic disorder. which means that its causes have not been found yet. However, a few processes, such is oxidative stress. protein aggregation and mitochondrial dysfunction are suspected to lead to the atrophy and death of substantia nigra (SN) neurons in case of this neurodegenerative disorder. Cu is a trace element whose role In the pathogenesis of PD is widely discussed. The investigation of Cu oxidation state inside single nerve cells from SN of PD and control cases may shed some new light oil the role of this element in PD. The differences in Cu chemical state were investigated with the use of X-ray absorption near edge structure (XANES) spectroscopy. The least-square fitting method was applied For the analysis or XANES spectra. The comparison of the positions of white line multiple scattering and pre-edge peak maximum at the energy scale did not reveal the existence of differences in Cu chemical state between PD and control samples. However, it was found that most of the Cu inside SN neurons occurs in tetrahedral environment and probably its Cu(II). (C) 2008 Elsevier GmbH. All rights reserved.