Effects of glucose and glucagon on the fructose 2,6-bisphosphate content of pancreatic islets and purified pancreatic B-cells. A comparison with isolated hepatocytes.
Effects of glucose and glucagon on the fructose 2,6-bisphosphate content of pancreatic islets and purified pancreatic B-cells. A comparison with isolated hepatocytes.
复制标题
葡萄糖和胰高血糖素对胰岛和纯化的胰腺 B 细胞中果糖 2,6-二磷酸含量的影响。
DOI：
10.1042/bj2210759
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Hers,HG
中科院分区：
文献类型：
--
作者：
Sener,A;VanSchaftingen,E;VandeWinkel,M;Pipeleers,DG;Malaisse-Lagae,F;Malaisse,WJ;Hers,HG
葡萄糖导致分离的胰岛以及纯化的胰腺 B 细胞的果糖 2,6-二磷酸含量持续且与剂量相关的增加。对于分离的 B 细胞，葡萄糖饱和曲线呈 S 形，并且与从未喂食的大鼠分离的肝细胞获得的曲线重叠。然而，纯化的 B 细胞对葡萄糖的反应明显快于分离的肝细胞。再次与肝脏中的情况相反，胰高血糖素未能显着降低胰岛或纯化的 B 细胞中果糖 2,6-二磷酸的浓度。有人提出，在葡萄糖刺激的胰岛素分泌过程中，果糖2,6-二磷酸形成的早期增加可能通过引起6-磷酸果糖-1-激酶的激活，使糖酵解与葡萄糖磷酸化的速率保持同步。
Glucose caused a sustained and dose-related increase in the fructose 2,6-bisphosphate content of isolated pancreatic islets, as well as of purified pancreatic B-cells. With isolated B-cells, the glucose saturation curve was sigmoidal and superimposable on that obtained with hepatocytes isolated from unfed rats. However, the response to glucose was notably faster in purified B-cells than in isolated hepatocytes. In contrast again with the situation prevailing in the liver, glucagon failed to decrease significantly the concentration of fructose 2,6-bisphosphate in either islets or purified B-cells. It is proposed that, in the process of glucose-stimulated insulin secretion, an early increase in fructose 2,6-bisphosphate formation may, by causing activation of 6-phosphofructo-1-kinase, allow glycolysis to keep pace with the rate of glucose phosphorylation.