The microRNA miR-8 is a positive regulator of pigmentation and eclosion in Drosophila.
The microRNA miR-8 is a positive regulator of pigmentation and eclosion in Drosophila.
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DOI：
10.1002/dvdy.23705
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发表时间：
2012-01
影响因子：
2.5
通讯作者：
Waldron, Evan J.
中科院分区：
文献类型：
--
作者：
Kennell, Jennifer A.;Cadigan, Ken M.;Shakhmantsir, Iryna;Waldron, Evan J.
MicroRNAs(MiRNAs)是一种短的、非编码的RNAs，通过与靶mRNAs结合，在转录后沉默基因的表达。以前的研究已经确定miRNA miR-8是果蝇发育的多效性调节因子，通过靶向多个mRNAs来控制机体大小和神经元存活。在这里，我们证明miR-8是成人腹部角质层上适当的色素空间图案所必需的。缺乏miR-8的雌性成虫表现出背腹色素减少，其色素沉积模式类似于在较高温度下生长的野生型果蝇。MiR-8突变体中的这种色素缺陷与先前报道的身体大小缺陷无关，并且miR-8直接作用于发育中的角质层来调节色素沉积模式。MiR-8的缺失增加了果蝇对色素形成和闭合能力的热敏感性。综上所述，这些数据表明miR-8在环境变化中起到了稳定基因表达模式的缓冲作用。
MicroRNAs (miRNAs) are short, non-coding RNAs that post-transcriptionally silence gene expression by binding to target mRNAs. Previous studies have identified the miRNA miR-8 as a pleiotropic regulator of Drosophila development, controlling body size and neuronal survival by targeting multiple mRNAs. Here we demonstrate that miR-8 is required for proper spatial patterning of pigment on the adult abdominal cuticle. Female adult flies lacking miR-8 exhibit decreased pigmentation of the dorsal abdomen, with a pattern of pigmentation similar to wild type flies grown at higher temperatures. This pigmentation defect in miR-8 mutants is independent of the previously reported body size defect, and miR-8 acts directly in the developing cuticle to regulate pigmentation patterning. Loss of miR-8 increases thermal sensitivity of Drosophila for both pigmentation patterning and the ability to eclose. Together, these data suggest miR-8 acts as a buffer to stabilize gene expression patterns in the midst of environmental variation.
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