Macroporous and nanofibrous poly(lactide-co-glycolide)(50/50) scaffolds via phase separation combined with particle-leaching
Macroporous and nanofibrous poly(lactide-co-glycolide)(50/50) scaffolds via phase separation combined with particle-leaching
复制标题
通过相分离结合颗粒浸出制备大孔和纳米纤维聚丙交酯乙交酯（50/50）支架
DOI：
10.1016/j.msec.2012.04.018
复制
发表时间：
2012-08-01
影响因子：
7.9
通讯作者：
Yang, Xiaoping
中科院分区：
文献类型：
--
作者：
Mao, Jifu;Duan, Shun;Yang, Xiaoping
聚丙交酯乙交酯 (PLGA) 共聚物是组织工程应用中最普遍的材料。为了模拟细胞生长的细胞外基质 (ECM) 的真实微环境，纳米纤维 PLGA 支架是首选。 PLGA5050（其中乳酰基与缩水甘油基单元的摩尔比为50:50）是一种完全无定形的聚合物，在本研究中首次报道利用PLGA5050/THF溶液的相分离将其制成纳米纤维网络（纤维直径约500 nm）。聚合物溶液的浓度对纤维直径和单位长度有显着影响。形成PLGA5050凝胶不需要非溶剂（例如H2O），这对于纳米纤维化至关重要，就好像发生凝胶化的环境温度足够低（-70℃）一样。稳定 PLGA5050/THF 凝胶的物理交联被认为是沿着主链的 GA 片段，因为它们在 THF 中的溶解度较差。由于缩水甘油基单元的亲水性，H2O的添加会导致液-液相分离和纳米纤维化失效的不利影响。结合相分离方法，采用颗粒浸出技术制备具有大孔和纳米纤维结构的三维支架。为了保证大孔壁纳米纤维化的发生，盐颗粒的温度最好预先降低至-70℃。因此，在不同参数下制备的支架表现出不同的纳米纤维和孔形态。其影响孔径、孔隙率、比表面积、水接触角和蛋白质吸附能力等。初步细胞（MC3T3-E1）培养证实，与单独的纳米纤维基质相比，细胞向大孔和纳米纤维PLGA5050支架内生长。这种双尺度三维矩阵可以成为组织工程应用的优质候选支架。 (C) 2012 Elsevier B.V. 保留所有权利。
Poly(lactide-co-glycolide) (PLGA) copolymers are the most prevalent materials for tissue engineering applications. To mimic the real microenvironment of extracellular matrix (ECM) for cell growth, nanofibrous PLGA scaffolds are preferred. PLGA5050 (in which the molar ratio of lactidyl to glycolidyl units is 50:50), which is an utterly amorphous polymer, was first reported to be made into nanofibrous networks (fiber diameter around 500 nm) using phase separation from PLGA5050/THF solutions in this study. The concentration of polymeric solution had significant effects on fiber diameter and unit length. Nonsolvent (e.g. H2O) was unnecessary to form the PLGA5050 gel, which was critical to nanofibrosis, as if the environmental temperature for gelation occurrence was low enough (-70 degrees C). The physical crosslinks to stabilize the PLGA5050/THF gel were believed to be GA segments along the backbone owing to their inferior solubility in THF. The addition of H2O would cause adverse effects of liquid-liquid phase separation and nanofibrosis failure owing to the hydrophilicity of glycolidyl units. Associating with the phase separation method, particle-leaching technique was applied to fabricate three-dimensional scaffolds with macroporous and nanofibrous structures. To ensure the occurrence of nanofibrosis on macropore walls, the temperature of salt particles should be best lowed to -70 degrees C beforehand. Accordingly, scaffolds prepared under varied parameters exhibited different nanofiber and pore morphologies. which affected the pore size, porosity, specific surface area, water contact angle and protein adsorption ability etc. The preliminary cell (MC3T3-E1) culture confirmed the cell ingrowth into the macroporous and nanofibrous PLGA5050 scaffolds in comparison with the solely nanofibrous matrixes. This kind of bi-scaled three dimensional matrixes can be superior candidate scaffolds for tissue engineering applications. (C) 2012 Elsevier B.V. All rights reserved.