AN ESSENTIAL GENE, ESR1, IS REQUIRED FOR MITOTIC CELL-GROWTH, DNA-REPAIR AND MEIOTIC RECOMBINATION IN SACCHAROMYCES-CEREVISIAE
AN ESSENTIAL GENE, ESR1, IS REQUIRED FOR MITOTIC CELL-GROWTH, DNA-REPAIR AND MEIOTIC RECOMBINATION IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1093/nar/22.15.3104
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发表时间：
1994-08-11
影响因子：
14.9
通讯作者：
OGAWA, H
中科院分区：
文献类型：
--
作者：
KATO, R;OGAWA, H
从酿酒酵母中分离到一株对甲基磺酸甲酯(MMS)和紫外光(UV)敏感、减数分裂重组缺陷的突变株。该基因ESR1是通过互补突变体的MMS敏感性而克隆的，被发现是细胞生长所必需的，因为缺失的单倍体菌株是致命的。ESR1基因与第二染色体上的CKS1基因相邻，编码一个2368个氨基酸的蛋白质，分子量为273k，转录产物全长8.0kb，在减数分裂过程中诱导表达。预测的ESR1蛋白具有由同源区域组成的镶嵌结构，并与监控DNA修复合成完成的裂殖酵母Rad3(+)蛋白和纺锤体极体(SPB)复制所需的ut1(+)蛋白的氨基酸序列相似。ESR1蛋白也类似于磷脂酰肌醇(PI)3-激酶，包括酿酒酵母TOR2(和DRR1)，它们参与了G(1)的进程。这些结果表明，ESR1在有丝分裂和减数分裂过程中是多功能的。
A new mutant, which was sensitive to both methyl-methanesulfonate (MMS) and ultra-violet light (UV) and defective in meiotic recombination, was isolated from Saccharomyces cerevisiae. The gene, ESR1, was cloned by complementation of the MMS sensitivity of the mutant and found to be essential for cell growth, as the deleted haploid strain was lethal. The ESR1 gene was adjacent to the CKS1 gene on chromosome II and encoded a putative 2368-amino acid protein with a molecular weight of 273 k. The ESR1 transcript was 8.0 kb long and was induced during meiosis. The predicted Esr1 protein had a mosaic structure composed of homologous regions and showed amino acid sequence similarities to Schizosaccharomyces pombe rad3(+) protein, which monitors completion of DNA repair synthesis, and cut1(+) protein, which is required for spindle pole body (SPB) duplication. The Esr1 protein was also similar to phosphatidylinositol (PI) 3-kinases, including Saccharomyces cerevisiae TOR2 (and DRR1), which are involved in G(1) progression. These results suggest that ESR1 is multi-functional throughout mitosis and meiosis.