Characterization of murine gammaherpesvirus 68 glycoprotein B
Characterization of murine gammaherpesvirus 68 glycoprotein B
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DOI：
10.1128/jvi.78.23.13370-13375.2004
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发表时间：
2004-12-01
影响因子：
5.4
通讯作者：
Stevenson, PG
中科院分区：
文献类型：
--
作者：
Lopes, FB;Colaco, S;Stevenson, PG
用免疫印迹、免疫沉淀和免疫电子显微镜等方法，从纯化的病毒粒子中鉴定出小鼠伽马疱疹病毒68(MHV-68)糖蛋白B(GB)。它在感染细胞中被合成为120 kDa的前体，并在接近病毒释放时被切割成65 kDa和55 kDa的二硫键连接的亚基。裂解的病毒粒子gB上的N-连接的糖链对内切糖苷酶H部分敏感。因此，MHV-68 GB的处理过程似乎类似于卡波西肉瘤相关疱疹病毒GB和人巨细胞病毒GB。
Murine gammaherpesvirus 68 (MHV-68) glycoprotein B (gB) was identified in purified virions by immunoblotting, immunoprecipitation, and immunoelectron microscopy. It was synthesized as a 120-kDa precursor in infected cells and cleaved into 65-kDa and 55-kDa disulfide-linked subunits close to the time of virion release. The N-linked glycans on the cleaved, virion gB remained partially endoglycosidase H sensitive. The processing of MHV-68 gB therefore appears similar to that of Kaposi's sarcoma-associated herpesvirus gB and human cytomegalovirus gB.