Human cells are permissive for the productive infection of porcine circovirus type 2 in vitro
Human cells are permissive for the productive infection of porcine circovirus type 2 in vitro
复制标题
人类细胞允许 2 型猪圆环病毒在体外进行有效感染
DOI：
10.1038/s41598-019-42210-0
复制
发表时间：
2019-04-04
影响因子：
4.6
通讯作者：
Ren, Linzhu
中科院分区：
文献类型：
--
作者：
Liu, Xiaohui;Ouyang, Ting;Ren, Linzhu
猪圆环病毒2型（Porcine Circovirus 2，PCV 2）是引起猪圆环病毒病和猪圆环病毒相关病的主要病原，在养猪国家普遍存在。然而，关于人类细胞对PCV 2感染的易感性存在争议。在这项研究中，人细胞系感染PCV 2并盲传数次。PCV 2进入人细胞并在其中复制，并产生感染性病毒体，表明人细胞系允许PCV 2复制。此外，PCV 2在人细胞系中的复制被D-葡糖胺或伴刀豆球蛋白A（ConA）增强。然而，PCV 2在人细胞中的感染效率低于PK-15细胞，表明PCV 2感染在人细胞中是有限的。我们的研究表明，人细胞是允许的生产性感染猪圆环病毒2型在体外。
Porcine circovirus 2 (PCV2) is the main pathogen of porcine circovirus diseases and porcine circovirus-associated diseases, which are widespread in swine-producing countries. However, there is controversy regarding the susceptibility of human cells to PCV2 infection. In this study, human cell lines were infected with PCV2 and blind passaged several times. PCV2 entered and replicated in human cells, and infectious virions were generated, indicating that human cell lines were permissive to PCV2 replication. Furthermore, PCV2 replication in human cell lines was enhanced by D-glucosamine or concanavalin A (ConA). However, the infection efficiency of PCV2 was lower in human cells than in PK-15 cells, suggesting that PCV2 infection was limited in human cells. Our study reveals that human cells are permissive for the productive infection of porcine circovirus type 2 in vitro.