Histological description of the midgut and the pyloric valve of Tropidacris collaris (Stoll, 1813) (Orthopetera: Romaleidae)
Histological description of the midgut and the pyloric valve of Tropidacris collaris (Stoll, 1813) (Orthopetera: Romaleidae)
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DOI：
10.1590/s1519-69842006000600011
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发表时间：
2006-11-01
影响因子：
--
通讯作者：
Oliveira, J. V.
中科院分区：
文献类型：
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作者：
Wanderley-Teixeira, V.;Teixeira, A. A. C.;Oliveira, J. V.
本研究描述了颈阔翅蝗Tropidacris collaris（Stoll，1813）（直翅目：短翅蝗科）中肠、胃盲囊和幽门瓣的组织学特征。我们使用光学显微镜，染色（Gomori三色和高碘酸希夫（PAS）），和常规组织学分析方法（苏木精-伊红）。昆虫获自伯南布哥农村联邦大学（UFRPE）生物系昆虫学实验室，并在该实验室中繁殖。收集的材料固定在酒精布因和包埋在paraplast。结果表明，中肠壁由一个内上皮层和两个外横纹肌层组成：一个内部（环形）和另一个外部（纵向），肌纤维之间有结缔组织。上皮是单层的，有两种细胞类型：再生和细长柱状。胃盲囊呈现与中肠相似的肌肉层。单层柱状上皮排列在胃盲囊上，呈现绒毛并向腔突出。幽门瓣由横纹肌组织构成，外覆单层上皮细胞。
The present research describes the histology of the midgut, gastric caeca, and pyloric valve of Tropidacris collaris (Stoll, 1813), (Orthopetera: Romaleidae). We used light microscopy, staining (Gomori's trichrome and periodic acid-Schiff (PAS)), and a routine histological analysis method (hematoxilin-eosin). The insects were obtained from, and also bred in, the Laboratory of Entomology, Department of Biology, of the Rural Federal University of Pernambuco (UFRPE). The collected material was fixed in alcoholic Bouin and embedded in paraplast. The results demonstrated that the midgut wall is composed of an inner epithelial layer and two outer layers of striate muscles: one internal (circular) and the other external (longitudinal), with connective tissue between the muscle fibers. The epithelium is single-layered, with two cell types: regenerative and elongated columnar. The gastric caeca presents muscle layers similar to those of the midgut. Simple columnar epithelium lines the gastric caeca, which presents villi and projects towards the lumen. The pyloric valve is of striate muscle tissue, covered by a single epithelial-cell layer.