The effect of human herpesvirus-6 (HHV-6) on cultured human neural cells:: oligodendrocytes and microglia
The effect of human herpesvirus-6 (HHV-6) on cultured human neural cells:: oligodendrocytes and microglia
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DOI：
10.3109/13550289809113493
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发表时间：
1998-10-01
影响因子：
3.2
通讯作者：
González-Scarano, F
中科院分区：
文献类型：
--
作者：
Albright, AV;Lavi, E;González-Scarano, F
Human herpesvirus-6 (HHV-6) is a betaherpesvirus that has been frequently associated with pediatric encephalitis. In 1995 Challoner et al reported that HHV-6 variant B (HHV-6B) was linked to multiple sclerosis (MS) due to the presence of viral DNA and antigen in the oligodendrocytes surrounding MS plaques. These findings led us to examine HHV-6B's in vitro tropism for primary neural cells. HIV-6B mediated cell-to-cell fusion in cultured adult oligodendroglia. Infection of oligodendrocytes was further confirmed by transmission electron microscopy (EM), which showed the presence of intracellular HHV-6 particles, and by PCR for HHV-6 DNA. However, the release of infectious virus was low or undetectable in multiple experiments. Microglia were also susceptible to infection by HHV-6B, as demonstrated by an antigen capture assay. We did not detect infection of a differentiated neuronal cell line (NT2D). Our findings suggest that HHV-6B infection of oligodendrocytes and/or microglia could potentially play a role in neuropathogenesis.
