Structural organization of the follicle-stimulating hormone receptor gene.
Structural organization of the follicle-stimulating hormone receptor gene.
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DOI：
10.1210/mend.6.1.1738373
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发表时间：
1992
影响因子：
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通讯作者：
L. Heckert;I J Daley;M. D. Griswold
中科院分区：
文献类型：
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作者：
L. Heckert;I J Daley;M. D. Griswold
我们已经表征了一系列大鼠基因组克隆，其编码FSH受体（FSHR）基因和转录起始位点上游约14.8个DNA酶。Southern杂交分析表明，FSHR基因只有一个。引物延伸和S1核酸酶实验揭示了在相对于翻译起始位点的位置-80和-98处存在两个主要的转录起始位点。含有830个碱基对的DNA 5'到翻译起始位点连接到报告基因氯霉素酰基转移酶的融合基因的瞬时表达研究表明，该基因的这一部分能够作为大鼠Sertoli细胞中的转录启动子。FSHR基因包含10个外显子和9个内含子。前9个外显子编码受体的广泛氨基末端结构域，而最后一个外显子编码跨膜和胞质结构域。在外显子2-9内描绘了类似于在富含亮氨酸的糖蛋白家族中观察到的重复基序。FSHR基因与LH受体基因的比较揭示了许多惊人的相似之处，这清楚地表明这些受体是通过基因复制进化而来的。这些受体的祖先基因大概是由富含亮氨酸的基序的一系列串联重复产生的，当与G蛋白偶联受体家族的共同祖先基因结合时，导致了糖蛋白激素受体的当前基因结构。
We have characterized a series of rat genomic clones that code for the FSH receptor (FSHR) gene and approximately 14.8 kilobases of DNA up-stream of the transcriptional start sites. Southern blot analysis indicated that there was only a single gene for the FSHR. Primer extension and S1 nuclease experiments revealed the presence of two major transcriptional start sites at positions -80 and -98 relative to the translational start site. Transient expression studies of a fusion gene containing 830 basepairs of DNA 5' to the translational start site linked to the reporter gene chloramphenicol acyltransferase have shown that this portion of the gene is capable of acting as a transcriptional promoter in rat Sertoli cells. The FSHR gene contained 10 exons and nine introns. The first nine exons encoded the extensive amino-terminal domain of the receptor, while the last exon encoded the transmembrane-spanning and cytoplasmic domains. A repeated motif similar to that observed in the leucine-rich glycoprotein family was delineated within exons 2-9. Comparison of the FSHR gene to the LH receptor gene revealed a number of striking similarities which clearly indicate that these receptors evolved through gene duplication. The ancestral gene for these receptors presumably arose from a series of tandem duplications of the leucine-rich motif, which when combined with the common ancestral gene of the G-protein-coupled receptor family led to the current gene structure of the glycoprotein hormone receptors.