Bioassay of serum opsonin and its depletion after colloid clearance in dogs.
Bioassay of serum opsonin and its depletion after colloid clearance in dogs.
复制标题
狗体内胶体清除后血清调理素及其消耗的生物测定。
DOI：
10.1152/ajplegacy.1972.223.3.569
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
T. Saba
中科院分区：
文献类型：
--
作者：
R. Cornell;T. Saba
题名/责任者：A.犬血清调理素的生物测定及其胶体清除后的耗竭。上午好。J.Physiol.223(3)：569-574。-利用胶化1311标记的RE-TEST-脂肪乳剂，研究了体外大鼠肝脏切片生物测定法测定异种狗血清中的调理或体液识别因子(HRF)活性以及调节肝脏吞噬功能的因素的有效性。大鼠肝巨噬细胞的吞噬功能受中等血清浓度、孵育时间和孵育温度的显著影响(P<；0.01)，在37℃下，当血清浓度超过50%时，摄取最大值出现在30min，与相应的血清研究相比，观察到明显的肝素依赖于狗血清对肝脏摄取的优化刺激。通过测定100、300和500 mg/kg剂量胶体清除犬血管内调理活动后循环中调理活动水平的时间变化，证明了调理素法的有效性和精确性。血清调理蛋白耗竭程度及其恢复率均与胶体剂量直接相关。恢复过程的动力学表明，体内存在两个光学蛋白质池，即“循环或血管内池”和“储存或血管外池”。肝脏清除；网状内皮功能和调节；肝素巨噬细胞或网状内皮系统(RES)在外来和排出的颗粒物质的生理清除和细胞内降解中的中心作用(2，4，7，32)，以及它参与免疫反应的传入弧线(11，12，14)的中心作用已有很好的文献记载。在这方面，巨噬细胞参与这种宿主防御活动高度依赖于它们迅速和选择性地吞噬有毒和/或抗原物质的能力。虽然吞噬作用已被证明受到代谢和循环稳定性等多种生化和生理因素的影响(2，7，32)，但大量证据表明，称为调色素(4，32，42)或体液识别因子(HRF)(28)的特定血清蛋白质的循环水平是RES(10，15，18，24，26，32，36，37)血管内吞噬作用的决定因素。Opsonic蛋白不仅增强吞噬过程，而且其活性可能在促进RE巨噬细胞的细胞内降解或杀菌活性方面起关键作用(16，17，31)。Saba和DiLuzio(33，37，38)最近的研究导致了一种用于快速测定血清调理或HRF(28)活性的体外组织切片生物测定法的发展。这项技术测量了患者的血清调理活性
CORNELL, ROBERT P., AND THOMAS M. SABA. Bioassay of serum opsonin and its depletion after colloid clearance in dogs. Am. J. Physiol. 223 (3): 569-574. 1972.-The validity of an in vitro rat liver slice biassay for the determination of opsonic or humoral recognition factor (HRF) activity in heterologous dog serum as well as factors regulating hepatic phagocytosis was investigated, utilizing the gelatinized 1311-labeled RE-test-lipid emulsion. Phagocytosis by rat hepatic macrophages was significantly (P< 0.01) altered by medium serum concentration, incubation duration, and incubation temperature, with maximal uptake occurring at a serum concentration above 50% by 30 min at 37 C. Comparable to homologous serum studies, a marked heparin dependency for the opsonic stimulation of hepatic uptake by dog serum was observed. The validity and precision of the opsonin bioassay was demonstrated by measuring the temporal changes in the circulating level of opsonic activity in dogs following intravascular colloid clearance utilizing doses of 100, 300, and 500 mg/kg body wt. Both the degree of serum opsonin depletion and its rate of restoration were directly and quantitatively related to the colloid dose. The kinetics of the recovery process suggested the presence of two pools of opsonic protein in the body, ie, a “circulating or intravascular pool” and a “storage or extravascular pool.” hepatic clearance; reticuloendothelial function and regulation; heparinTHE CENTRAL ROLE of the macrophage or reticuloendothelial system (RES) in the physiologic clearance and intracellular degradation of foreign and effete particulate matter (2, 4, 7, 32) as well its involvement in the afferent arc of the immune response (11, 12, 14) is well documented. In this regard, participation of macrophages in such host defense activities is highly dependent on their ability to rapidly and selectively phagocytize toxic and/or antigenic material. While phagocytosis has been shown to be influenced by a variety of biochemical and physiologic factors such as metabolic and circulatory stability (2, 7, 32), substantial evidence indicates that the circulating level of specific serum proteins called opsonins (4, 32, 42) or humoral recognition factors (HRF)(28) is a determinant of intravascular phagocytosis by the RES (10, 15, 18, 24, 26, 32, 36, 37). Opsonic protein not only enhances the phagocytic process, but its activity may also be critical in promoting the intracellular degradative or bactericidal activity of RE macrophages(16, 17, 3 1). Recent studies by Saba and DiLuzio(33, 37, 38) have led to the development of an in vitro tissue slice bioassay for the rapid determination of serum opsonic or HRF (28) activity. This technique measures serum opsonic activity in