Coating morphology and surface composition of acrylic terpolymers with pendant catechol, OEG and perfluoroalkyl groups in varying ratio and the effect on protein adsorption
Coating morphology and surface composition of acrylic terpolymers with pendant catechol, OEG and perfluoroalkyl groups in varying ratio and the effect on protein adsorption
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不同比例的邻苯二酚、OEG和全氟烷基丙烯酸三元共聚物的涂层形貌和表面组成以及对蛋白质吸附的影响
DOI：
10.1016/j.colsurfb.2015.12.051
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Aidong Zhang
中科院分区：
文献类型：
--
作者：
Jun Zhong;Hua Ji;Jiang Duan;Haiyang Tu;Aidong Zhang
这项工作的目的是利用带有不同比例的儿茶酚 (D)、低聚乙二醇 (O) 和全氟烷基 (F) 基团的丙烯酸三元共聚物 (DOF) 开发多功能低生物污垢聚合物涂层。这些聚合物能够在几乎任何表面上形成牢固的涂层，并进行表面微相分离和自组装，F 吊坠的表面富集以及从不规则固体域到不同尺寸的离散火山口型聚集体的形态变化表明了这一点。使用牛血清白蛋白（BSA）和粘附纤维蛋白原（Fib）作为模型蛋白质研究对蛋白质吸附的影响。 DOF164（低氟含量）涂层具有约 400 nm 大小的离散弹坑型聚集体形态，通过 SPR 和免疫吸附测定测定，吸附的蛋白质量最少，但蛋白质单位活性最高；而DOF1612（高氟含量）涂层对Fib的吸附能力高出12.3倍。有趣的是，发现吸附在 DOF164 表面上的 Fib 比吸附在 DOF250（不含 F 组分）上的吸附量低 2.2 倍，但单位活性高 1.8 倍，而 DOF250 的 OEG 片段是广泛认可的蛋白质相容性材料。 DOF164三元共聚物的特点是具有强大的涂层能力和最小的蛋白质吸附能力，同时具有高蛋白质单位活性，这表明其具有作为医用防污涂层的防污表面改性剂以及作为生物传感器构建中选择性蛋白质固定和活性保留的基质材料的潜在应用。
This work aims at developing versatile low-biofouling polymeric coatings by using acrylic terpolymers(DOFs) that bear pendant catechol (D), oligo(ethylene glycol) (O), and perfluoroalkyl (F) groups in varying ratios. The polymers were endowed with the ability to form firmly coatings on virtually any surfaces and undergo surface microphase separation and self-assembly, as revealed by the surface enrichment of F pendants and the morphology variation from irregular solid domains to discrete crater-type aggregates of different size. The effect on protein adsorption was investigated using bovine serum albumin (BSA) and adhesive fibrinogen (Fib) as model proteins. The coating of DOF164 (low F content), which has morphology of discrete crater-type aggregates of ∼400 nm in size, adsorbed a least amount of protein but with a highest protein unit activity as determined by SPR and immunosorbent assay; whereas the coating ofDOF1612 (high F content) showed a 12.3-fold higher adsorption capacity toward Fib. Interestingly, a 2.2-fold lower adsorption amount but with a 1.8-fold higher unit activity was found for Fib adsorbed on theDOF164 surface than on DOF250 (without F fraction), whose OEG segments being a widely recognized protein compatible material. The features of the DOF164 terpolymer presenting a robust coating ability and a minimal protein adsorption capacity while with a high protein unit activity suggest its potential application as a non-fouling surface-modifier for medical antifouling coatings and as a matrix material for selective protein immobilization and activity preservation in biosensor construction.