FLUORESCENCE DETECTION IN AUTOMATED DNA-SEQUENCE ANALYSIS
FLUORESCENCE DETECTION IN AUTOMATED DNA-SEQUENCE ANALYSIS
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DOI：
10.1038/321674a0
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发表时间：
1986-06-12
期刊：
影响因子：
64.8
通讯作者：
HOOD, LE
中科院分区：
文献类型：
--
作者：
SMITH, LM;SANDERS, JZ;HOOD, LE
我们开发了一种 DNA 序列分析部分自动化的方法。 DNA 片段的荧光检测是通过共价连接到用于酶促 DNA 序列分析的寡核苷酸引物的荧光团来完成的。针对碱基 A、C、G 和 T 的每个反应均使用不同颜色的荧光团。将反应混合物合并并在单个聚丙烯酰胺凝胶管中进行共电泳，在管底部附近检测分离的 DNA 荧光带，并通过计算机直接获取序列信息。
We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated flourescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer.