ESTRADIOL-INDUCED PROGESTIN RECEPTOR IMMUNOREACTIVITY IS FOUND ONLY IN ESTROGEN RECEPTOR-IMMUNOREACTIVE CELLS IN GUINEA-PIG BRAIN
ESTRADIOL-INDUCED PROGESTIN RECEPTOR IMMUNOREACTIVITY IS FOUND ONLY IN ESTROGEN RECEPTOR-IMMUNOREACTIVE CELLS IN GUINEA-PIG BRAIN
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DOI：
10.1159/000125152
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发表时间：
1989-05-01
影响因子：
4.1
通讯作者：
TURCOTTE, JC
中科院分区：
文献类型：
--
作者：
BLAUSTEIN, JD;TURCOTTE, JC
荧光免疫细胞化学技术的发展，以确定是否在豚鼠下丘脑和视前区的细胞，含有雌二醇诱导的雌激素受体也含有雌激素受体。用这种技术，很少或没有观察到雌激素治疗的情况下，卵巢切除豚鼠孕酮受体免疫反应性（PR-IR）。如先前所报道的，用雌二醇引发引起下丘脑和视前区的离散区域中PR-IR细胞浓度的大幅增加，主要是在弓状核、下丘脑的腹外侧区、室周视前区、内侧视前核、内侧视前区、下丘脑前核和下丘脑前区。在这些区域中的每一个中观察到一系列高浓度的轻度至强烈标记的雌激素受体免疫反应（ER-IR）细胞，以及在一些未鉴定出PR-IR细胞的区域中，如杏仁核。PR-IR仅见于同时具有ER-IR的细胞中，在下丘脑腹外侧区和弓状核等部位，几乎所有的ER-IR中强荧光细胞也含有雌二醇诱导的PR-IR，而杏仁核中尽管ER-IR细胞浓度较高，但未观察到PR-IR。这些结果证实了这一假设，即雌激素受体是在大脑中含有雌激素受体的细胞中产生的，并且它们表明这些细胞是雌二醇和孕酮影响行为和生理的部位。
A fluorescent immunocytochemical technique was developed to determine if cells in the guinea pig hypothalamus and preoptic area that contain estradiol-induced progestin receptors also contain estrogen receptors. With this technique little or no progestin receptor-immunoreactivity (PR-IR) was observed in the absence of estrogen treatment in ovariectomized guinea pigs. As has been reported previously, priming with estradiol caused a large increase in the concentration of PR-IR cells in discrete regions of the hypothalamus and preoptic area, primarily in the arcuate nucleus, ventrolateal area of the hypothalamus, periventricular preoptic area, medial preoptic nucleus, medial preoptic area, anterior hypothalamic nucleus and anterior hypothalamus. A range of lightly to intensely labeled estrogen receptor-immunoreactive (ER-IR) cells were observed in high concentration in each of these areas, as well as in some areas in which no PR-IR cells have been identified, such as the amygdala. PR-IR was only observed in cells that also had ER-IR. In some areas such as the ventrolateral hypothalamic area and arcuate nucleus, nearly all medium to highly-fluorescent ER-IR cells also contained estradiol-induced PR-IR, while in the amygdala no PR-IR was observed despite a high concentration of ER-IR cells. These results confirm the hypothesis that progestin receptors are produced in estrogen receptor-containing cells in the brain, and they suggest that these cells are the sites where estradiol and progesterone act to influence behavior and physiology.