Localization of nonspecific lipid transfer protein (nsLTP = sterol carrier protein 2) and acyl-CoA oxidase in peroxisomes of pigment epithelial cells of rat retina.
Localization of nonspecific lipid transfer protein (nsLTP = sterol carrier protein 2) and acyl-CoA oxidase in peroxisomes of pigment epithelial cells of rat retina.
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大鼠视网膜色素上皮细胞过氧化物酶体中非特异性脂质转移蛋白（nsLTP = 甾醇载体蛋白 2）和酰基辅酶 A 氧化酶的定位。
DOI：
--
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发表时间：
1992
影响因子：
3.2
通讯作者：
Yutaka Tashiro
中科院分区：
文献类型：
--
作者：
J. Deguchi;Akitsugu Yamamoto;Y. Fujiki;Masanobu Uyama;I. Tsukahara;Yutaka Tashiro
应用免疫金标法和抗生物素-生物素-过氧化物酶-亲和素复合法，观察了非特异性脂转移蛋白(NsLTP)在大鼠视网膜，特别是色素上皮(RPE)细胞中的定位。光镜观察发现，视网膜色素上皮、内节层、神经纤维层和Müler细胞均含有nsLTP。在RPE细胞中，金颗粒仅集中在小的过氧体(直径0.1-0.3微米的微过氧体)中，并用抗nsLTP和抗过氧化氢酶抗体进行双重染色。在过氧化体中，金颗粒均匀地分布在基质中，没有观察到与限制膜的优先结合。酰辅酶A氧化酶也定位于过氧化体的基质中。我们认为，RPE细胞中的过氧酶体在外节盘膜的脂质代谢中起着重要作用，特别是在多不饱和长链和超长链脂肪酸的β-氧化过程中，如二十二碳六烯酸，它约占盘膜中脂肪酸的三分之一。
We investigated the localization of nonspecific lipid transfer protein (nsLTP) in rat retina, especially in the pigment epithelial (RPE) cells, by the avidin-biotin-peroxidase complex method on cryosections for light microscopy and by the cryoimmunogold method for electron microscopy. Light microscopic observation revealed that the RPE, inner segment layer, nerve fiber layer, and Müller cells contain nsLTP. In the RPE cells gold particles were exclusively concentrated in the small peroxisomes (microperoxisomes; 0.1-0.3 micron in diameter), which were identified by double staining using anti-nsLTP and anti-catalase antibodies. In the peroxisomes gold particles were distributed homogeneously in the matrices and no preferential binding to the limiting membrane was observed. Acyl-CoA oxidase was also localized in the matrices of the peroxisomes. We suggest that the peroxisomes in RPE cells play important roles in the metabolism of lipids of the outer segment disk membranes, especially in the beta-oxidation of polyunsaturated long-chain and very long-chain fatty acids, such as docosahexaenoic acid which is composed of approximately one third of fatty acids in the disk membranes.
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omega-3 多不饱和脂肪酸在眼睛中的代谢：二十二碳六烯酸和二十二烷酸在视网膜生理学和眼部病理学中的可能作用。
DOI：
--
发表时间：
1989
期刊：
Progress in clinical and biological research
影响因子：
--
作者：
Bazan,NG
通讯作者：
Bazan,NG
肝和肾皮质中脂肪酸代谢热稳定和热不稳定烯酰辅酶 A (CoA) 水合酶的免疫细胞化学定位。
DOI：
10.1177/31.4.6338108
发表时间：
1983
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Bendayan,M;Reddy,MK;Hashimoto,T;Reddy,JK
通讯作者：
Reddy,JK
影响因子：
4.4
作者：
Bok,D
通讯作者：
Bok,D
甾醇载体和脂质转移蛋白。
DOI：
10.1016/b978-0-12-024922-0.50007-2
发表时间：
1987
期刊：
Advances in lipid research
影响因子：
--
作者：
Vahouny,GV;Chanderbhan,R;Kharroubi,A;Noland,BJ;Pastuszyn,A;Scallen,TJ
通讯作者：
Scallen,TJ