Suppression of lactate production of Lactobacillus reuteri JCM1112 by co-feeding glycerol with glucose
Suppression of lactate production of Lactobacillus reuteri JCM1112 by co-feeding glycerol with glucose
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DOI：
10.1016/j.jbiosc.2019.07.004
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发表时间：
2020-01-01
影响因子：
2.8
通讯作者：
Katakura, Yoshio
中科院分区：
文献类型：
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作者：
Ichinose, Ryo;Fukuda, Yuichi;Katakura, Yoshio
乳酸菌产生的乳酸抑制其生长。为了抑制乳酸的产生，有必要通过乳酸脱氢酶以外的替代途径来再生糖酵解消耗的 NAD+。在异质发酵乳酸菌罗伊氏乳杆菌 JCM1112 中，研究了在从甘油生产 1,3-丙二醇时通过再生 NAD+ 来抑制乳酸生产。该细菌在使用葡萄糖作为唯一碳源的分批培养中产生乳酸，产量为 4.7 +/- 0.8 g.g-cell(-1)。当在分批培养中以摩尔比 (r(Gly/Glc)) 将甘油添加到葡萄糖中时，细菌以 1.6 +/- 0.7 g.g-cell(-1).h(-1) 产生 1,3-丙二醇，并且乳酸产量降低至 3.6 +/- 0.5 g.g-cell(-1)。当甘油与葡萄糖呈指数共饲以达到 0.1 h(-1) (r(Gly/Glc) = 3) 的目标比生长速率时，乳酸产量降低至 1.5 +/- 0.2 g.g-cell(-1)。当甘油与葡萄糖一起添加时，乳酸产量减少至使用葡萄糖作为碳源的分批培养中观察到的乳酸产量的三分之一。 (C) 2019 年，日本生物技术协会。版权所有。
Lactate produced by lactic acid bacteria inhibits their growth. To suppress lactate production, it is necessary to regenerate NAD+ consumed by glycolysis with alternative pathways other than lactate dehydrogenase. In a heterofermentative lactic acid bacterium, Lactobacillus reuteri JCM1112, suppression of lactate production by regenerating NAD+ when producing 1,3-propanediol from glycerol was investigated. The bacterium produced lactate with a yield of 4.7 +/- 0.8 g.g-cell(-1) in a batch culture using glucose as the sole carbon source. When glycerol was added to glucose at a molar ratio (r(Gly/Glc)) of three in the batch culture, the bacterium produced 1,3-propanediol at 1.6 +/- 0.7 g.g-cell(-1).h(-1) and the lactate yield decreased to 3.6 +/- 0.5 g.g-cell(-1). When glycerol was co-fed with glucose exponentially to give a target specific growth rate of 0.1 h(-1) (r(Gly/Glc) = 3), the lactate yield decreased to 1.5 +/- 0.2 g.g-cell(-1). The lactate production when glycerol was added together with glucose was reduced to one-third of that observed in the batch culture using glucose as a carbon source. (C) 2019, The Society for Biotechnology, Japan. All rights reserved.