In vitro construction of scaffold-free cylindrical cartilage using cell sheet-based tissue engineering
In vitro construction of scaffold-free cylindrical cartilage using cell sheet-based tissue engineering
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DOI：
10.1007/s00383-009-2543-3
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发表时间：
2010-02-01
影响因子：
1.8
通讯作者：
Fukuzawa, Masahiro
中科院分区：
文献类型：
--
作者：
Tani, Gakuto;Usui, Noriaki;Fukuzawa, Masahiro
组织工程软骨可能为严重气道疾病的治疗提供解决方案。本研究开发了一种新的程序，在体外条件下制造无支架的圆柱形软骨，同时也评估了动态培养对工程construct. Aar软骨细胞新西兰白色兔和高密度条件下培养，形成软骨细胞片的效果。将该片材环绕在硅管上，并在动态或静态条件下培养6周。肉眼和组织学评价工程圆柱软骨。胶原蛋白的表达，糖胺聚糖含量和机械性能进行了测定。圆柱形软骨有足够的弹性和刚度，以保持结构不被破坏。组织学上，该结构含有番红-O阳性软骨基质，伴随着II型胶原蛋白的表达。培养6周后，气管软骨中糖胺聚糖含量增加，达到天然气管软骨的72%。这个过程可以允许定制的自体移植物和功能工程气管的发展。
Tissue-engineered cartilage may offer a solution for the treatment of serious airway disease. This study developed a novel procedure to fabricate a scaffold-free cylindrical cartilage under in vitro conditions, while also evaluating the effect of a dynamic culture on the engineered construct.Auricular chondrocytes were harvested from New Zealand white rabbits and cultivated under high-density conditions to form a chondrocyte sheet. The sheet was looped around a silicon tube and cultivated for 6 weeks in dynamic or static conditions. The engineered cylindrical cartilages were evaluated macroscopically and histologically. The expression of collagen, glycosaminoglycan content and mechanical properties were determined.The cylindrical cartilage was sufficiently elastic and stiff to maintain the structure without disruption. Histologically, the construct contained a Safranin-O positive cartilaginous matrix accompanied by the expression of type II collagen. The glycosaminoglycan content increased and reached 72% of the native tracheal cartilage after 6 weeks of cultivation.A novel procedure was developed for fabricating engineered cartilage, which maintained the shape and a proper level of rigidity and flexibility, under in vitro conditions using sheet-based tissue engineering techniques. This procedure may allow for the development of a tailor-made autograft and a functionally engineered trachea.