INVITRO AND INVIVO COMPARATIVE COLONIZATION OF STAPHYLOCOCCUS-AUREUS AND STAPHYLOCOCCUS-EPIDERMIDIS ON ORTHOPEDIC IMPLANT MATERIALS
INVITRO AND INVIVO COMPARATIVE COLONIZATION OF STAPHYLOCOCCUS-AUREUS AND STAPHYLOCOCCUS-EPIDERMIDIS ON ORTHOPEDIC IMPLANT MATERIALS
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DOI：
10.1016/0142-9612(89)90073-2
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发表时间：
1989-07-01
期刊：
影响因子：
14
通讯作者：
GRISTINA, AG
中科院分区：
文献类型：
--
作者：
BARTH, E;MYRVIK, QM;GRISTINA, AG
临床上，金黄色葡萄球菌似乎是与感染的金属植入物相关的主要微生物，而凝固酶阴性葡萄球菌菌株更频繁地从感染的聚合物植入物中分离出来。我们在体外和体内[兔子]实验中重现了这一趋势。将钛合金、聚（甲基丙烯酸甲酯）和超高分子量聚乙烯制成的椎间盘暴露于金黄色葡萄球菌临床分离株或两种表皮葡萄球菌菌株中的任一种。在1小时内，无论生物材料如何，金黄色葡萄球菌始终是最快速的定殖者。然而，在8至24小时后，金黄色葡萄球菌在金属上以较高的数量存在，表皮葡萄球菌在聚合物上以较高的数量存在。此外，表皮葡萄球菌产生的胞外多糖似乎提供了有效的保护，在体内抵抗宿主防御。
Clinically, Staphylococcus aureus appears to be the dominant organism associated with infected metal implants, whereas coagulase-negative staphylococcal strains are more frequently isolated from infected polymer implants. We reproduced this trend experimentally in vitro and in vivo [rabbits]. Disc of a titanium alloy, poly(methyl methacrylate) and ultra-high molecular weight polyethylene were exposed to a clinical isolate of Staphylococcus aureaus or either of two strains of Staphylococcus epidermidis. Within 1 h Staphylococcus aureus was always the most rapid colonizer regardless of biomaterial. However, after 8 to 24 h, Staphylococcus aureus was present in higher numbers on metal and Staphylococcus epidermidis on polymers. Moreover, the exopolysaccharide produced by Staphylococcus epidermidis appeared to offer an effective protection against host defences in vivo.