Isolation and Molecular Characterization of the Transformer Gene From Bactrocera cucurbitae (Diptera: Tephritidae).
Isolation and Molecular Characterization of the Transformer Gene From Bactrocera cucurbitae (Diptera: Tephritidae).
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DOI：
10.1093/jisesa/iex031
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Yan R
中科院分区：
文献类型：
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作者：
Luo Y;Zhao S;Li J;Li P;Yan R
Transformer（tra）基因是许多昆虫，特别是双翅目昆虫的性别决定开关基因。然而，瓜实蝇（Bactrocera cucurbitae，Coquillett）的性别决定途径，地球上一个非常具有破坏性的害虫，仍然在很大程度上是未知的。在这项研究中，我们已经分离和特点的一个女性特异性和两个男性特异性转录的tra基因（Bcutra）的B。葫芦科Bcutra基因组结构分析表明，Bcutra基因组中存在多个保守的Transformer（TRA）/TRA-2结合位点，Bcutra与其它实蝇科果蝇的同源基因高度保守。Bcutra在不同发育阶段的基因表达分析表明，Bcutra的雌性转录本出现早于雄性转录本，表明母体TRA在卵中遗传，并可能在TRA表达的调控中起作用。Bcutra蛋白序列的保守性和性别特异性剪接以及在发育过程中的表达模式表明，Bcutra可能是南瓜B性别决定的主基因。Bcutra的分离将有助于开发用于生物防治的遗传性别鉴定菌株。
transformer (tra) is a switch gene of sex determination in many insects, particularly in Dipterans. However, the sex determination pathway in Bactrocera cucurbitae (Coquillett), a very destructive pest on earth, remains largely uncharacterized. In this study, we have isolated and characterized one female-specific and two male-specific transcripts of the tra gene (Bcutra) of B. cucurbitae. The genomic structure of Bcutra has been determined and the presence of multiple conserved Transformer (TRA)/TRA-2 binding sites in Bcutra has been found. BcuTRA is highly conservative with its homologues in other tephritid fruit flies. Gene expression analysis of Bcutra at different developmental stages demonstrates that the female transcript of Bcutra appears earlier than the male counterparts, indicating that the maternal TRA is inherited in eggs and might play a role in the regulation of TRA expression. The conservation of protein sequence and sex-specific splicing of Bcutra and its expression patterns during development suggest that Bcutra is probably the master gene of sex determination of B. cucurbitae. Isolation of Bcutra will facilitate the development of a genetic sexing strain for its biological control.