Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells.
Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells.
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分析人和小鼠原代肥大细胞中 1-磷酸鞘氨醇信号传导的方法。
DOI：
10.1007/7651_2017_42
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Oskeritzian,CaroleA
中科院分区：
文献类型：
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作者：
Chumanevich,AlenaP;Wedman,PiperA;Oskeritzian,CaroleA
肥大细胞组成性地和在激活时产生有效生物活性的鞘脂代谢物鞘氨醇-1-磷酸（S1 P）。S1 P与肥大细胞上的2型受体的连接触发了一种新的下游信号传导途径，我们发现该途径将转录因子信号转导子和转录激活因子3的激活与人类和小鼠中肥大细胞衍生的趋化因子释放联系起来。在这一章中，我们描述了用于研究S1 P信号在人类和小鼠原代肥大细胞的方法。
Mast cells produce a potently bioactive sphingolipid metabolite sphingosine-1-phosphate (S1P) constitutively and upon activation. The ligation of S1P to its type 2 receptor on mast cells triggers a novel downstream signaling pathway that we discovered links activation of transcription factor signal transducer and activator of transcription 3 to mast cell-derived chemokine release in both humans and mice. In this chapter, we describe the methods used to study S1P signaling in human and mouse primary mast cells.