Spontaneous basophil responses in food-allergic children are transferable by plasma and are IgE-dependent.
Spontaneous basophil responses in food-allergic children are transferable by plasma and are IgE-dependent.
复制标题
食物过敏儿童的自发嗜碱性粒细胞反应可通过血浆转移，并且具有 IgE 依赖性。
DOI：
10.1016/j.jaci.2013.08.033
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Frischmeyer-Guerrerio,PamelaA
中科院分区：
文献类型：
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作者：
Schroeder,JohnT;Bieneman,AnjaP;Chichester,KristinL;Keet,CorinneA;Hamilton,RobertG;MacGlashanJr,DonaldW;Wood,Robert;Frischmeyer-Guerrerio,PamelaA
早期研究表明，高达80%的食物过敏儿童表现出高自发嗜碱性粒细胞组胺释放（SBHR）。[1]由于食物过敏（FA）儿童的嗜碱性粒细胞额外释放组胺至IgE依赖性组胺释放因子，因此提示与IgE相关。2然而，对SBHR的确切机制，包括对IgE的依赖性，仍然知之甚少。我们最近报道，接受舌下免疫治疗/口服免疫治疗的儿童SBHR显著降低，（SLIT/OIT）用于牛奶过敏，3促使进一步研究这种反应和嗜碱性粒细胞高反应性的其他标志物。（所有牛奶）和18名非过敏儿童，如本文在线知识库的方法部分所述，可在www. jacionline。24名儿童在基线和SLIT/OIT方案期间接受了随访。3因此，在此对该亚组中研究的几个嗜碱性粒细胞参数的基线值进行了重述，以证明先前未报道的相关性。关于所有受试者的支持性人口统计学和临床信息总结在本文在线知识库的表E1中，可在www. jacionline。牛奶过敏的诊断是基于对牛奶的急性反应性（rxn+）和超过0.10 KUA/L（cap+）的牛奶特异性IgE水平的令人信服的病史。所有FA受试者在抽血时严格避免牛奶。对照组受试者没有对任何食物（rxn−）的急性反应史，从未避免任何食物，目前可以耐受饮食中的牛奶。尽管18名对照受试者中有3名确实有低水平的牛奶特异性IgE（最高为1.52 KUA/L），但低于FA组（中位数，52.5 KUA/L;范围，1.9-1108.0 KUA/L）。
Early studies showed that up to 80% of children with food allergy demonstrate high spontaneous basophil histamine release (SBHR). 1 An association with IgE was suggested because basophils from food-allergic (FA) children additionally released histamine to the IgE-dependent histamine-releasing factor. 2 However, the exact mechanism (s) responsible for SBHR, including the dependency for IgE, remains poorly understood. We recently reported that SBHR significantly decreased in children undergoing sublingual immunotherapy/oral immunotherapy (SLIT/OIT) for cow's milk allergy, 3 prompting further investigations of this response and other markers of basophil hyperresponsiveness.Basophils were isolated from the blood of 38 FA (all to cow's milk) and 18 nonallergic children, as described in the Methods section of this article's Online Repository available at www. jacionline. org. Twenty-four children had been followed at baseline and during the SLIT/OIT protocol. 3 Thus, baseline values for several basophil parameters investigated in this subgroup are restated herein to demonstrate correlative associations not previously reported. Supporting demographic and clinical information regarding all subjects is summarized in Table E1 of this article's Online Repository available at www. jacionline. org. Diagnosis of milk allergy was based on a convincing history of acute reactivity to cow's milk (rxn+) and a milk-specific IgE level of more than 0.10 KUA/L (cap+). All FA subjects were strictly avoiding milk at the time blood was drawn. Control subjects had no history of acute reaction to any food (rxn−), had never avoided any foods, and were currently tolerating milk in their diet. Although, 3 of the 18 control subjects did have low levels of milk-specific IgE (1.52 KUA/L being the highest), these were less than those in the FA group (median, 52.5 KUA/L; range, 1.9-1108.0 KUA/L).