Simultaneous recording of breathing and neural activity in awake behaving mice.
Simultaneous recording of breathing and neural activity in awake behaving mice.
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同时记录清醒行为小鼠的呼吸和神经活动。
DOI：
10.1016/j.xpro.2022.101412
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发表时间：
2022-06-17
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该协议提供了一个管道，同时记录呼吸和神经活动清醒，行为小鼠。呼吸通过植入鼻腔的热敏电阻探针来记录，该探针可以轻松与纤维光度测定等神经活动监测方法集成。在这里，我们详细介绍了热敏电阻探头组装，手术，记录系统设置和数据分析的程序。该方案可应用于研究呼吸生理学和自然行为期间的呼吸变化。有关本方案使用和执行的完整详细信息，请参阅。构建一个简单、轻便的呼吸传感器的方案传感器植入手术和行为设置说明提高信号质量和稳定性的提示灵活地与多个记录和操作平台集成出版商说明：进行任何实验方案都需要遵守当地机构的实验室安全和道德准则。该协议为同时记录清醒、行为正常的小鼠的呼吸和神经活动提供了一个管道。呼吸通过植入鼻腔的热敏电阻探针来记录，该探针可以轻松与纤维光度测定等神经活动监测方法集成。在这里，我们详细介绍了热敏电阻探头组装，手术，记录系统设置和数据分析的程序。该方案可应用于研究呼吸生理学和自然行为期间的呼吸变化。
This protocol provides a pipeline for simultaneous recording of breathing and neural activities in awake, behaving mice. Breathing is recorded with thermistor probes implanted in the nasal cavity, which can be easily integrated with neural activity monitoring approaches such as fiber photometry. Here, we detail the procedures of the thermistor probe assembly, surgery, recording system setup, and data analysis. This protocol can be applied to investigate respiratory physiology and breathing changes during natural behaviors. For complete details on the use and execution of this protocol, please refer to. Protocols for constructing a simple, lightweight breathing sensor Instructions for sensor implantation surgery and behavior setup Tips on improving the signal quality and stability Flexibility to integrate with multiple recording and manipulation platforms Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. This protocol provides a pipeline for simultaneous recording of breathing and neural activities in awake, behaving mice. Breathing is recorded with thermistor probes implanted in the nasal cavity, which can be easily integrated with neural activity monitoring approaches such as fiber photometry. Here, we detail the procedures of the thermistor probe assembly, surgery, recording system setup, and data analysis. This protocol can be applied to investigate respiratory physiology and breathing changes during natural behaviors.