Conformational activation of visual rhodopsin in native disc membranes
Conformational activation of visual rhodopsin in native disc membranes
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DOI：
10.1126/scisignal.2005646
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发表时间：
2015-03-10
影响因子：
7.3
通讯作者：
Neutze, Richard
中科院分区：
文献类型：
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作者：
Malmerberg, Erik;Bovee-Geurts, Petra H. M.;Neutze, Richard
视紫红质是一种G蛋白偶联受体（GPCR），在脊椎动物中作为一种昏暗的视觉受体。我们利用时间分辨广角x射线散射技术研究了室温下紫红质在天然膜环境下的光诱导构象变化。我们观察到一个快速的构象转变，这与跨膜螺旋6的细胞质部分向外倾斜与跨膜螺旋5的细胞质部分向内移动是一致的。这些运动比根据激活的视紫红质的晶体结构报道的运动要大得多，这意味着光激活视紫红质的构象变化比以前认为的更广泛。
Rhodopsin is the G protein-coupled receptor (GPCR) that serves as a dim-light receptor for vision in vertebrates. We probed light-induced conformational changes in rhodopsin in its native membrane environment at room temperature using time-resolved wide-angle x-ray scattering. We observed a rapid conformational transition that is consistent with an outward tilt of the cytoplasmic portion of transmembrane helix 6 concomitant with an inward movement of the cytoplasmic portion of transmembrane helix 5. These movements were considerably larger than those reported from the basis of crystal structures of activated rhodopsin, implying that light activation of rhodopsin involves a more extended conformational change than was previously suggested.