Selective Detection of Target Proteins by Peptide-Enabled Graphene Biosensor
Selective Detection of Target Proteins by Peptide-Enabled Graphene Biosensor
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DOI：
10.1002/smll.201302188
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发表时间：
2014-04-01
期刊：
影响因子：
13.3
通讯作者：
Sarikaya, Mehmet
中科院分区：
文献类型：
--
作者：
Khatayevich, Dmitriy;Page, Tamon;Sarikaya, Mehmet
生物标志物的直接分子检测是诊断和监测多种疾病的一种很有前途的方法，也是现代分子医学和药物发现的基石。目前，生物标志物的临床应用受到现有间接检测方法的敏感性、复杂性和低选择性的限制。电子一维和二维纳米材料，如碳纳米管和石墨烯，分别为简单、快速和超灵敏的生物分子结合检测提供了独特的优势。然而，多功能方法尚未开发用于传感器表面的同时功能化和钝化，以增强设备的检测和选择性。在此，我们展示了使用石墨烯传感器通过自组装多功能短肽功能化，在血清蛋白背景下对模型蛋白的选择性检测。这两种肽被设计成与石墨烯结合，并在衬底上以有序的单分子膜的形式进行共组装。当探针肽显示生物活性分子时，钝化肽阻止非特异性蛋白质吸附到器件表面，确保目标选择性。特别是，我们展示了一种石墨烯场效应晶体管（gFET）生物传感器，它可以在低于50 ng/ml的血清牛白蛋白背景下检测链霉亲和素。我们的纳米传感器设计，使我们能够恢复石墨烯表面，并在多个实验中使用每个传感器。肽激活的gFET装置具有解决各种生物传感问题的巨大潜力，例如研究配体-受体相互作用，或在临床环境中检测生物标志物。
Direct molecular detection of biomarkers is a promising approach for diagnosis and monitoring of numerous diseases, as well as a cornerstone of modern molecular medicine and drug discovery. Currently, clinical applications of biomarkers are limited by the sensitivity, complexity and low selectivity of available indirect detection methods. Electronic 1D and 2D nano-materials such as carbon nanotubes and graphene, respectively, offer unique advantages as sensing substrates for simple, fast and ultrasensitive detection of biomolecular binding. Versatile methods, however, have yet to be developed for simultaneous functionalization and passivation of the sensor surface to allow for enhanced detection and selectivity of the device. Herein, we demonstrate selective detection of a model protein against a background of serum protein using a graphene sensor functionalized via self-assembling multifunctional short peptides. The two peptides are engineered to bind to graphene and undergo co-assembly in the form of an ordered monomolecular film on the substrate. While the probe peptide displays the bioactive molecule, the passivating peptide prevents non-specific protein adsorption onto the device surface, ensuring target selectivity. In particular, we demonstrate a graphene field effect transistor (gFET) biosensor which can detect streptavidin against a background of serum bovine albumin at less than 50 ng/ml. Our nano-sensor design, allows us to restore the graphene surface and utilize each sensor in multiple experiments. The peptide-enabled gFET device has great potential to address a variety of bio-sensing problems, such as studying ligand-receptor interactions, or detection of biomarkers in a clinical setting.