Content of K+ and Na+ in seminiferous tubule and rete testis fluids from Sertoli cell-enriched testes.
Content of K+ and Na+ in seminiferous tubule and rete testis fluids from Sertoli cell-enriched testes.
复制标题
来自富含支持细胞的睾丸的生精小管和睾丸网液中 K 和 Na 的含量。
DOI：
10.1095/biolreprod33.5.1245
复制
发表时间：
1985
影响因子：
3.6
通讯作者：
Hall,PF
中科院分区：
文献类型：
--
作者：
Muffly,KE;Turner,TT;Brown,M;Hall,PF
Seminiferous tubules of rats exposed to x-irradiation before birth were subjected to micropuncture in situ at 50 days of age to obtain samples of fluid 4 h after ligation of efferent ducts. The concentrations of cations in this fluid were: potassium, 39.7 ± 1.2 mM, and sodium, 136.3 ± 1.2 mM (means and standard errors,n= 5). Histologic examination revealed that germ cells constitute less than 1% of the cell population within the seminiferous tubules of these rats; the remaining cells were all Sertoli cells. Sertoli cells showed efflux of86Rb+witht1/2of approximately 11 min and an active ATPase in plasma membranes. These activities were similar to those of Sertoli cells from normal rats. Germ cells from normal rats showed less rapid efflux of86Rb+(t1/2> 60 min) and less active Na+/K+ATPase in plasma membranes. It is concluded that Sertoli cells are responsible for the high concentration of potassium in seminiferous tubule fluid and that plasma membranes of these cells contain an active K+pump that is not inhibited by ouabain (1 mM).
