Correlation of ultrasound-induced hemolysis with cavitation detector output in vitro
Correlation of ultrasound-induced hemolysis with cavitation detector output in vitro
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DOI：
10.1016/s0301-5629(97)00039-2
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发表时间：
1997-01-01
影响因子：
2.9
通讯作者：
Meltzer, RS
中科院分区：
文献类型：
--
作者：
Everbach, EC;Makin, IRS;Meltzer, RS
使用20 MHz被动声探测器对强脉冲超声作用下样品中发生的瞬时声空化量进行了定量，体外将含有和不含有微泡回声造影剂的人红细胞稀释液暴露于中心频率为1 MHz、声脉冲持续时间为20、100、200、500和1000 mU的500W/cm(SPPA)超声中，脉冲重复频率为20 Hz。通过探测器输出的时间平均值测量样本内发生的惯性空化与溶血有很好的相关性，这表明气泡的剧烈坍塌是细胞损伤的原因，这一结果也提出了空化监测作为临床使用回声造影剂时不良生物效应的可能预测指标的前景。(C)1997年世界医学和生物学超声联合会。
A 20-MHz passive acoustic detector was used to quantify the amount of transient acoustic cavitation occurring in a sample exposed to intense pulsed ultrasound, A dilute suspension of human erythrocytes with and without a microbubble echo-contrast agent was exposed in vitro to 500 W/cm(2) (SPPA) ultrasound of center frequency 1 MHz and tone burst duration 20, 100, 200, 500 and 1000 mu s at a pulse repetition frequency of 20 Hz. Inertial cavitation occurring within the sample, as measured by the temporal average of the detector output, correlated well with hemolysis, suggesting that violent bubble collapse is responsible for cell damage, The result also raises the prospect of cavitation monitoring as a possible predictor of adverse bioeffects when echo-contrast agents are used clinically. (C) 1997 World Federation for Ultrasound in Medicine & Biology.