Optimizing recovery of peptides from an octadecylsilyl (ODS) cartridge.
Optimizing recovery of peptides from an octadecylsilyl (ODS) cartridge.
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优化十八烷基甲硅烷基 (ODS) 柱中肽的回收率。
DOI：
10.1111/j.1399-3011.1989.tb01278.x
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Desiderio,DM
中科院分区：
文献类型：
--
作者：
Higa,T;Desiderio,DM
详细研究了一种分析方法，以优化从反相十八烷基硅基一次性药筒中回收放射性标记和未标记的合成肽物质P和蛋氨酸-脑啡肽的方法，这是从生物基质中优先富集肽的第一步。我们之前对生物多肽的研究表明了本研究的必要性。发现柱流率是最重要的实验参数之一；采用高效液相色谱法测定其对两种合成多肽的回收率，并采用高效液相色谱法测定其对合成多肽回收率的影响。流量的准确性和重现性影响回收率，有必要调节注射器输液泵的流量。使用慢速样品应用和洗脱回收率最佳。此外，缓慢的洗脱比缓慢的样品应用速度更重要。在从生物基质中提取肽之前，需要对单个合成肽进行这种基本的恢复研究。
An analytical procedure has been studied in detail to optimize the recovery of the radiolabeled and unlabeled synthetic peptides substance P and methionine‐enkephalin from a reversed phase octadecylsilyl disposable cartridge, which is one of the first steps to preferentially enrich peptides from a biologic matrix. Our previous research on biologic peptides indicated the need for the present study. The column flow‐rate was found to be one of the most important experimental parameters; and its effect on the recovery of those two synthetic peptides was determined by the measurement of recovered radioactivity and by high performance liquid chromatography U.V. detection. The accuracy and reproducibility of the flow‐rate affected recovery, and it was necessary to regulate the flow‐rate with a syringe infusion pump. Recovery was optimum using a slow rate for both sample application and elution. Furthermore, a slow elution was more important than a slow rate of sample application. Such basic recovery studies on individual synthetic peptides are required before undertaking peptide extractions from biologic matrices.