Dynamic recruitment of dynamin for final mitochondrial severance in a primitive red alga
Dynamic recruitment of dynamin for final mitochondrial severance in a primitive red alga
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DOI：
10.1073/pnas.0436886100
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发表时间：
2003-02-18
影响因子：
11.1
通讯作者：
Kuroiwa, T
中科院分区：
文献类型：
--
作者：
Nishida, K;Takahara, M;Kuroiwa, T
发动蛋白是真核生物特异性的GTP酶家族。一些家庭成员参与各种各样的细胞活动。在这里，我们报告说，原始的红色Cyanidioschyzon merolae保留只有一个发动蛋白同系物，CmDnm 1，属于线粒体分裂亚家族。以前，细菌细胞分裂蛋白，FtsZ，被证明定位在线粒体分裂位点的细菌。我们发现，FtsZ和发动蛋白作为线粒体分裂相关蛋白共存，在分裂的不同阶段发挥作用。CmDnm 1从10-20个细胞质斑块（发动蛋白斑块）募集到收缩的细胞分裂环（MD环）的中点，观察到细胞质侧的电子致密结构。CmDnm 1可能不是早期收缩所必需的;当线粒体外膜最终被切断时，它形成一个环或螺旋，而FtsZ和MD环在收缩前形成。据认为，FtsZ，MD和发动蛋白环分别参与支架，收缩和最终分离。在真核生物中，线粒体断裂可能是动力蛋白家族最保守的作用。
Dynamins are a eukaryote-specific family of GTPases. Some family members are involved in diverse and varied cellular activities. Here, we report that the primitive red alga Cyanidioschyzon merolae retains only one dynamin homolog, CmDnm1, belonging to the mitochondrial division subfamily. Previously, the bacterial cell division protein, FtsZ, was shown to localize at the mitochondrial division site in the alga. We showed that FtsZ and dynamin coexist as mitochondrial division-associated proteins that act during different phases of division. CmDnm1 was recruited from 10-20 cytoplasmic patches (dynamin patches) to the midpoint of the constricted mitochondrion-dividing ring (MD ring), which was observed as an electron-dense structure on the cytoplasmic side. CmDnm1 is probably not required for early constriction; it forms a ring or spiral when the outer mitochondrial membrane is finally severed, whereas the FtsZ and MD rings are formed before constriction. It is thought that the FtsZ, MD, and dynamin rings are involved in scaffolding, constriction, and final separation, respectively. In eukaryotes, mitochondrial severance is probably the most conserved role for the dynamin family.