Urinary MicroRNAs as Potential Markers for Non-Invasive Diagnosis of Bladder Cancer
Urinary MicroRNAs as Potential Markers for Non-Invasive Diagnosis of Bladder Cancer
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DOI：
10.3390/ijms21113814
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发表时间：
2020-06-01
影响因子：
5.6
通讯作者：
Fuessel, Susanne
中科院分区：
文献类型：
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作者：
Erdmann, Kati;Salomo, Karsten;Fuessel, Susanne
目前，排尿细胞学（VUC）是检测尿液中膀胱癌（BCa）的金标准。尽管 VUC 具有很高的特异性，但在敏感性方面存在缺陷。因此，正在寻找替代的生物标志物，这可能会克服这些缺点，作为 VUC 的有用辅助手段。本研究的目的是评估尿液中选定的 microRNA (miR) 水平的诊断潜力，由于其 BCa 特异性表达，这些微小 RNA (miR) 可能代表此类替代生物标志物。通过定量 PCR 评估 104 名原发性 BCa 患者和 46 名对照受试者的尿沉渣中 9 种 BCa 相关 microRNA（miR-21、-96、-125b、-126、-145、-183、-205、-210、-221）的表达水平。受试者工作特征 (ROC) 曲线分析显示 miR-96、-125b、-126、-145、-183 和 -221 具有诊断潜力，曲线下面积 (AUC) 值在 0.605 至 0.772 之间。四个最佳候选者的组合产生的敏感性、特异性、阳性和阴性预测值 (NPV) 以及准确性分别为 73.1%、95.7%、97.4%、61.1% 和 80.0%。与VUC结合使用，灵敏度和NPV可提高近8%，均超过单独使用VUC的性能。目前的研究结果表明，miR-125b、-145、-183 和 -221 与 VUC 结合用于尿液中 BCa 的无创检测具有诊断潜力。
Currently, voided urine cytology (VUC) serves as the gold standard for the detection of bladder cancer (BCa) in urine. Despite its high specificity, VUC has shortcomings in terms of sensitivity. Therefore, alternative biomarkers are being searched, which might overcome these disadvantages as a useful adjunct to VUC. The aim of this study was to evaluate the diagnostic potential of the urinary levels of selected microRNAs (miRs), which might represent such alternative biomarkers due to their BCa-specific expression. Expression levels of nine BCa-associated microRNAs (miR-21, -96, -125b, -126, -145, -183, -205, -210, -221) were assessed by quantitative PCR in urine sediments from 104 patients with primary BCa and 46 control subjects. Receiver operating characteristic (ROC) curve analyses revealed a diagnostic potential for miR-96, -125b, -126, -145, -183, and -221 with area under the curve (AUC) values between 0.605 and 0.772. The combination of the four best candidates resulted in sensitivity, specificity, positive and negative predictive values (NPV), and accuracy of 73.1%, 95.7%, 97.4%, 61.1%, and 80.0%, respectively. Combined with VUC, sensitivity and NPV could be increased by nearly 8%, each surpassing the performance of VUC alone. The present findings suggested a diagnostic potential of miR-125b, -145, -183, and -221 in combination with VUC for non-invasive detection of BCa in urine.