Integration of molecular genetics and proteomics with cell metabolism: How to proceed; How not to proceed!
Integration of molecular genetics and proteomics with cell metabolism: How to proceed; How not to proceed!
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DOI：
10.1016/j.gene.2011.06.035
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发表时间：
2011-10-15
期刊：
影响因子：
3.5
通讯作者：
Franklin, Renty B.
中科院分区：
文献类型：
--
作者：
Costello, Leslie C.;Franklin, Renty B.
现在人们对中间代谢在医学中的作用重新产生了兴趣；尤其是在医学疾病方面。与此相结合的是当代对分子生物学、遗传学和蛋白质组学的关注，以及它们与健康和疾病中细胞代谢的调节和改变的研究的整合。这一结合在阐明细胞代谢和功能变化所涉及的因素和条件方面具有巨大的潜力，而在此之前，建立了中间代谢主要途径的前几代生物化学家还无法解决这些问题。实现这一目前的潜力需要适当的应用和解释有关细胞代谢和细胞功能的遗传和蛋白质组学研究。这需要对原理、关系和方法的知识和理解，如生物化学和酶学，这些都涉及到细胞调节酶和代谢途径的阐明。不幸的是，许多，可能是大多数当代分子生物学家在细胞代谢的这些领域没有得到足够的训练和知识。这导致了细胞代谢和功能的遗传/蛋白质组学研究中太常见的不适当应用和错误信息。本报告描述了细胞中间代谢的重要关系，并提供了适当和不适当的应用遗传学和蛋白质组学的例子。它呼吁在分子生物学和其他生物医学研究人员的研究生和研究生培训中纳入生物化学、酶学、细胞代谢和细胞生理学。(C) 2011 Elsevier B.V.版权所有
There now exists a resurgence of interest in the role of intermediary metabolism in medicine; especially in relation to medical disorders. Coupled with this is the contemporary focus on molecular biology, genetics and proteomics and their integration into studies of regulation and alterations in cellular metabolism in health and disease. This is a marriage that has vast potential for elucidation of the factors and conditions that are involved in cellular metabolic and functional changes, which heretofore could not be addressed by the earlier generations of biochemists who established the major pathways of intermediary metabolism. The achievement of this present potential requires the appropriate application and interpretation of genetic and proteomic studies relating to cell metabolism and cell function. This requires knowledge and understanding of the principles, relationships, and methodology, such as biochemistry and enzymology, which are involved in the elucidation of cellular regulatory enzymes and metabolic pathways. Unfortunately, many and possibly most contemporary molecular biologists are not adequately trained and knowledgeable in these areas of cell metabolism. This has resulted in much too common inappropriate application and misinformation from genetic/proteomic studies of cell metabolism and function. This presentation describes important relationships of cellular intermediary metabolism, and provides examples of the appropriate and inappropriate application of genetics and proteomics. It calls for the inclusion of biochemistry, enzymology, cell metabolism and cell physiology in the graduate and postgraduate training of molecular biology and other biomedical researchers. (C) 2011 Elsevier B.V. All rights reserved.