Optimization of Low-Cost Culture Media for the Production of Biomass and Bacteriocin by a Urogenital Lactobacillus salivarius Strain
Optimization of Low-Cost Culture Media for the Production of Biomass and Bacteriocin by a Urogenital Lactobacillus salivarius Strain
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DOI：
10.1007/s12602-010-9037-4
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发表时间：
2010-03-01
影响因子：
4.9
通讯作者：
Nader-Macias, Maria Elena F.
中科院分区：
文献类型：
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作者：
Juarez Tomas, Maria Silvina;Bru, Elena;Nader-Macias, Maria Elena F.
本工作的目的是配制一种比传统实验室培养基成本更低的培养基，以便同时获得阴道唾液乳杆菌CRL 1328的高生物量和细菌素。采用2（8-2）中心复合实验设计（有一个中心点和16个附加点）在不同培养条件下进行生长测定。考虑的因素是葡萄糖、乳糖、酵母提取物、胰蛋白胨、柠檬酸铵、乙酸钠、MgSO4 和MnSO4。碳源（主要是葡萄糖）、氮源（主要是酵母提取物）和盐（主要是 MnSO4、MgSO4 和乙酸钠）的同时存在使得实验设计中能够达到最高的细胞生物量和细菌素水平。通过应用期望函数，预测了实现生物质和细菌素高效生产的几种最佳培养基组成。与传统肉汤相比，优化的生长培养基可降低约 25% 至 40% 的成本。所获得的结果代表了在寻找最合适的生产用于预防或治疗女性泌尿生殖感染的药品的生物活性化合物的策略方面取得了进展。
The aim of this work was to formulate a culture medium of lower cost than conventional laboratory media, in order to simultaneously obtain high amounts of both biomass and bacteriocin of vaginal Lactobacillus salivarius CRL 1328. The growth assays under different culture conditions were performed by using a 2(8-2) central composite experimental design, with a central point and sixteen additional points. The factors taken into consideration were glucose, lactose, yeast extract, tryptone, ammonium citrate, sodium acetate, MgSO4 and MnSO4. The simultaneous presence of a carbon source (mainly glucose), a nitrogen source (mainly yeast extract) and salts (mainly MnSO4, MgSO4 and sodium acetate) allowed the highest cell biomass and bacteriocin levels to be reached in the experimental design. Through the application of the desirability function, several optimal medium compositions to achieve efficient production of biomass and bacteriocin were predicted. The optimized growth media allow a cost reduction of around 25 to 40% compared with conventional broths. The results obtained represent an advance in the search of the most suitable strategies for the production of bioactive compounds for pharmaceutical products to prevent or treat female urogenital infections.