Reversible Fusion Proteins as a Tool to Enhance Uptake of Virus-Functionalized LbL Microcarriers.
Reversible Fusion Proteins as a Tool to Enhance Uptake of Virus-Functionalized LbL Microcarriers.
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DOI：
10.1021/acs.biomac.8b00360
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发表时间：
2018-07
影响因子：
6.2
通讯作者：
Kira Scheffler;C. Claus;M. Stanifer;S. Boulant;U. Reibetanz
中科院分区：
文献类型：
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作者：
Kira Scheffler;C. Claus;M. Stanifer;S. Boulant;U. Reibetanz
为了有效治疗越来越多的疾病，开发新的治疗方法以及新的药物递送系统是必不可少的。这类给药系统（DDS）不仅要考虑生物降解性和保护性包装，而且还必须靶向和控制活性物质的释放，这是DDS应用中最重要的一点。我们强调了这些关键方面的改进，即在用水泡性口炎病毒（VSV）功能化后，逐层（LbL）设计的微载体作为一种有前途的DDS的相互作用率增加。我们利用VSV融合蛋白独特的构象可逆性作为LbL微载体的表面功能化。这种可逆性允许VSV既用作组装到DDS上的工具，又用作有效细胞摄取的引发剂。我们可以证明，进化优化的病毒融合机制可以成功地与生物物理DDS相结合，以优化其细胞相互作用。
For the efficient treatment of an increasing number of diseases the development of new therapeutics as well as novel drug delivery systems is essential. Such drug delivery systems (DDS) must not only consider biodegradability and protective packaging but must also target and control the release of active substances, which is one of the most important points in DDS application. We highlight the improvement of these key aspects, the increased interaction rate of Layer-by-Layer (LbL) designed microcarriers as a promising DDS after functionalization with vesicular stomatitis virus (VSV). We make use of the unique conformational reversibility of the fusion protein of VSV as a surface functionalization of LbL microcarriers. This reversibility allows for VSV to be used both as a tool for assembly onto the DDS and as an initiator for an efficient cellular uptake. We could show that the evolutionary optimized viral fusion machinery can be successfully combined with a biophysical DDS for optimization of its cellular interaction.