Resistance mechanism of Fusarium fujikuroi to phenamacril in the field
Resistance mechanism of Fusarium fujikuroi to phenamacril in the field
复制标题
藤黑镰孢菌对非那丙烯利的田间抗性机制
DOI：
10.1002/ps.4742
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发表时间：
2018-03-01
影响因子：
4.1
通讯作者：
Zhou, Ming-Guo
中科院分区：
文献类型：
--
作者：
Hou, Yi-Ping;Qu, Xiang-Pu;Zhou, Ming-Guo
背景：水稻bakanae病是一种重要的水稻病害，主要由Fusarium fujikuroi引起。在中国，非那马克利用于控制该病已有几年的历史。2016年浙江省野外共发现耐药菌株9株。本研究的目的是阐明fujikuroi对非那马克利的耐药机制和耐药菌株的适合度。结果：9株fujikuroi菌株对非那马克利高度耐药。其中8例在Myosin-5蛋白密码子219位点发生点突变TCA (Ser) CCA (Pro)，另1例在密码子219位点发生点突变TCA (Ser) TTA （Leu）。耐药菌株和敏感菌株之间肌球蛋白-5的替换证实了肌球蛋白-5的点突变引起了fujikuroi F.对非那马克利的耐药。将非那马克利与Myosin-5模型结合袋对接，结果表明，非那马克利与Myosin-5的亲和力下降，当非那马克利变为Pro或Leu后，非那马克利与密码子219处的氨基酸不能形成氢键，非那马克利与其他杀菌剂无交叉抗性。含有点突变S219P的8株抗性菌株与敏感菌株的适应度基本相同，而含有点突变S219 L的1株抗性菌株的菌丝生长、产孢量和致病性均下降。结论：在田间试验中，肌球蛋白-5位点突变S219P或s219l使富士黑僵菌对非那马那利产生了高耐药性。点突变S219P不影响赤霉病的适应度，而点突变S219 L降低了赤霉病的适应度。(C) 2017年化学工业学会
BACKGROUND: Rice bakanae disease, mainly caused by Fusarium fujikuroi, is an important disease of rice. Phenamacril has been used to control the disease for a few years in China. In 2016, nine phenamacril-resistant strains were found in the field in Zhejiang Province. The aim of the study was to clarify the mechanism of resistance of F. fujikuroi to phenamacril and the fitness of resistant strains.RESULTS: The nine F. fujikuroi strains examined were highly resistant to phenamacril. Eight of them had the point mutation TCA (Ser) CCA (Pro) at codon 219 in the Myosin-5 protein, while the other had the point mutation TCA (Ser) TTA (Leu) at codon 219. Myosin-5 replacement between resistant and sensitive strains confirmed that the point mutation in Myosin-5 caused the resistance of F. fujikuroi to phenamacril. Docking of phenamacril into the modeled binding pocket of Myosin-5 showed that the affinity between phenamacril and Myosin-5 decreased and a hydrogen bond could not be formed between phenamacril and the amino acid at codon 219 after it changed to Pro or Leu. There was no cross-resistance between phenamacril and other fungicides. The eight resistant strains containing the point mutation S219P had almost the same fitness as the sensitive strains, while the one resistant strain containing the point mutation S219 L showed decreased mycelial growth, sporulation and pathogenicity.CONCLUSION: In the field, the point mutation S219P or S219 L in Myosin-5 conferred high resistance to phenamacril in F. fujikuroi. The point mutation S219P did not affect the fitness of F. fujikuroi, while the point mutation S219 L decreased its fitness. (C) 2017 Society of Chemical Industry