Failure to activate interleukin 1beta-converting enzyme-like proteases and to cleave retinoblastoma protein in drug-resistant cells.
Failure to activate interleukin 1beta-converting enzyme-like proteases and to cleave retinoblastoma protein in drug-resistant cells.
复制标题
无法激活白细胞介素 1β 转换酶样蛋白酶，也无法裂解耐药细胞中的视网膜母细胞瘤蛋白。
DOI：
10.1016/s0014-5793(96)01311-7
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发表时间：
1996
期刊：
影响因子：
3.5
通讯作者：
Dou,QP
中科院分区：
文献类型：
--
作者：
An,B;Jin,JR;Lin,P;Dou,QP
We previously found that retinoblastoma (RB) is cleaved at the initiation of apoptotic execution. Here we report that when an HL-60 cell line resistant to cytosine arabinoside (Ara-C) was exposed to this anticancer drug, neither RB cleavage nor apoptosis was detected. Consistent with that, processing of interleukin 1β-converting enzyme (ICE) and CPP32 (an ICE-like protease) was also prevented in these cells. In contrast, treatment of the HL-60-Ara-C-resistant cells with etoposide induced all of these apoptotic events. Furthermore, the etoposide-induced RB cleavage was inhibited by a specific tetrapeptide ICE-like inhibitor. Our results demonstrate that activation of the RB cleavage enzyme, an ICE-like protease, is required for overcoming drug resistance.
