Polyherbal mixture ameliorates hyperglycemia, hyperlipidemia and histopathological changes of pancreas, kidney and liver in a rat model of type 1 diabetes
Polyherbal mixture ameliorates hyperglycemia, hyperlipidemia and histopathological changes of pancreas, kidney and liver in a rat model of type 1 diabetes
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DOI：
10.1016/j.jep.2020.113210
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发表时间：
2021-01-30
影响因子：
5.4
通讯作者：
Vasiljevic, Perica
中科院分区：
文献类型：
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作者：
Madic, Visnja;Petrovic, Aleksandra;Vasiljevic, Perica
民族药理作用：欧洲草药学家常用的抗糖尿病中药混合物之一是由悬钩子和越橘叶、垂根委陵菜根、金银花地上部分和菜豆豆荚制成的。目的：本研究旨在评价该中药混合物的植物化学成分、抗氧化能力、潜在毒性、降血糖、降血脂、保护肾脏和保肝活性。材料和方法：采用高效液相-紫外检测法对其植物化学成分进行评价。用DPPH试验评价其抗氧化活性。采用急性和亚慢性经口毒性方法评价其潜在毒性。Wistar雌性大鼠一次性腹腔注射一水合四氧嘧啶(150 mg/kg)建立糖尿病模型。连续14天血糖<20 mmoL/L者为糖尿病动物。在接下来的14天里，D-10和D-20组分别给予该中药混合物(分别为10和20g/kg的干植物材料)。I组和M组分别给予甘精胰岛素(13IU/kg)和二甲双胍(150 mg/kg)。健康对照组(HC)和糖尿病对照组(DC)给予水处理。分别于第14、21、28天测定血糖水平。于第28天进行血脂谱分析。用HE染色和Masson‘s三色染色评价胰腺、肾脏和肝脏的组织病理学改变。结果：肝组织中含有奎尼酸、没食子酸和咖啡酸、熊果苷、芦丁、三叶皂苷、黄芪甲苷、金丝桃苷、异槲皮苷和槲皮苷。提取物的抗氧化活性高于参照物(p<；0.01)。用多草药混合物(10和20g/kg)治疗没有显示出毒性作用。与DC组相比，I组和M组的血糖无明显下降(分别为22.86+/-2.58、28.5+/-0.42和27.82+/-0.9 mmol/L)。将血糖降至正常值(D-10、D-20和HC组分别为8.64±4.09、5.26+/-1.3和6.76+/-1.54 mmol/L)。此外，它还能降低总胆固醇、甘油三酯、极低密度脂蛋白、低密度脂蛋白、动脉粥样硬化和心血管危险指数(p
Ethnopharmacological relevance: One of the commonly prescribed 'anti-diabetic' polyherbal mixtures by European herbalists is made of Rubus fruticosus and Vaccinium myrtillus leaves, Potentilla erecta roots, Geum urbanum aerial parts and Phaseolus vulgaris pods.Aim of the study: This study aimed to evaluate the phytochemical composition, antioxidant capacity, potential toxicity, hypoglycemic, hypolipidemic, nephroprotective and hepatopmtective activities of this polyherbal mixture decoction.Materials and methods: The phytochemical composition was evaluated using HPLC-UV. The antioxidant activity was assessed using the DPPH test. Potential toxicity was evaluated using the acute and sub-chronic oral toxicity method. Diabetes was induced in Wistar female rats with a single intraperitoneal injection of alloxan monohydrate (150 mg/kg). The animals whose blood glucose was >20 mmol/L for 14 consecutive days were considered diabetic. For the next 14 days, D-10 and D-20 groups were treated with the polyherbal mixture (10 and 20 g of dry plant material/kg, respectively). I and M were control groups treated with insulin glargine (13 IU/kg) and metformin (150 mg/kg), respectively. Healthy control (HC) and diabetic control (DC) groups were treated with water. The blood glucose level was measured on days 14, 21 and 28. Lipid profile analysis was done on day 28. Pancreas, kidney and liver histopathology was evaluated using the H&E and Masson's trichrome staining. The liver tissue was additionally tested for PAS-positive cells.Results: The HPLC-UV analysis revealed the presence of quinic, gallic and caftaric acid, arbutin, rutin, trifolin, astragalin, hyperoside, isoquercetin and quercitrin. The antioxidant activity of the extract was higher than the reference's one (p < 0.01). Treatment with the polyherbal mixture (10 and 20 g/kg) has shown no toxic effects. No major decline in blood sugar was recorded in I and M groups compared to the DC one (22.86 +/- 2.58, 28.5 +/- 0.42 and 27.82 +/- 0.9 mmol/L, respectively). The polyherbal mixture lowered the blood glucose level to the normal value (8.64 + 4.09, 5.26 +/- 1.3 and 6.76 +/- 1.54 mmol/L in D-10, D-20 and HC groups, respectively). Furthermore, it decreased the levels of total cholesterol, triglycerides, VLDL, LDL, atherogenic and cardiovascular risk indices (p