Identification and characterization of RPTPρ, a novel RPTPμ/κ-like receptor protein tyrosine phosphatase whose expression is restricted to the central nervous system
Identification and characterization of RPTPρ, a novel RPTPμ/κ-like receptor protein tyrosine phosphatase whose expression is restricted to the central nervous system
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DOI：
10.1016/s0169-328x(98)00014-x
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发表时间：
1998-05-01
期刊：
影响因子：
--
通讯作者：
Burghes, AHM
中科院分区：
文献类型：
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作者：
McAndrew, PE;Frostholm, A;Burghes, AHM
我们描述了 RPTP rho（RPTP mu/kappa 磷酸酶亚家族的新成员）的克隆、染色体定位和表征。该亚家族中的受体酪氨酸磷酸酶由 N 端附近的 MAM 结构域、免疫球蛋白样结构域、四个纤连蛋白 III 型重复序列、一个跨膜结构域和一个大的近膜片段组成，后跟两个细胞内磷酸酶结构域。在 RPTP rho 的细胞外片段中，在第四个纤连蛋白 II 型重复序列和跨膜结构域之间，发现了一个可变剪接的微型外显子。 RPTR rho 基因定位于人类 20 号染色体和小鼠 2 号染色体，Northern blot 分析表明 RPTP rho 表达仅限于中枢神经系统，原位杂交研究表明 RPTP rho 转录本分布于整个小鼠大脑和脊髓。在围产期发育期间，皮质和嗅球中存在异常高水平的转录本，但在出生后两周内下调。 II型受体蛋白酪氨酸磷酸酶胞外片段中发现的基序常见于神经细胞粘附分子中，表明RPTP rho可能参与中枢神经系统中的信号转导和细胞粘附。 (C) 1998 Elsevier Science B.V.
We describe the cloning, chromosomal localization and characterization of RPTP rho, a new member of the RPTP mu/kappa phosphatase subfamily. Receptor tyrosine phosphatases in this subfamily are comprised of a MAM domain near the N-terminal, an immunoglobulin-like domain, four fibronectin type III repeats, a single transmembrane domain, and a large juxtamembrane segment followed by two intracellular phosphatase domains. An alternatively spliced mini-exon was identified in the extracellular segment of RPTP rho, between the fourth fibronectin type II repeat and the transmembrane domain. The RPTR rho gene was mapped to human chromosome 20 and mouse chromosome 2, Northern blot analysis demonstrated that RPTP rho expression was restricted to the central nervous system, and in situ hybridization studies showed that the RPTP rho transcript was distributed throughout the murine brain and spinal cord. Exceptionally high levels of the transcript were present in the cortex and olfactory bulbs during perinatal development, but were down-regulated during postnatal week two. The motifs found in the extracellular segment of type II receptor protein tyrosine phosphatases are commonly found in neural cell adhesion molecules, suggesting that RPTP rho may be involved in both signal transduction and cellular adhesion in the central nervous system. (C) 1998 Elsevier Science B.V.