Generation of Drosophila Heparan Sulfate Mutant Cell Lines from Existing Fly Strains.
Generation of Drosophila Heparan Sulfate Mutant Cell Lines from Existing Fly Strains.
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DOI：
10.1007/978-1-0716-1398-6_47
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Nakato H
中科院分区：
文献类型：
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作者：
Nakato E;Bowden N;Nakato H
利用模式生物黑腹果蝇进行的遗传学研究有助于阐明硫酸乙酰肝素蛋白聚糖（HSPGs）的体内功能。另一方面，果蝇糖胺聚糖（GAG）的生物化学分析受到限制，主要是由于从动物中获得的材料量不足。最近，一种新的体外系统已被开发，通过建立突变的细胞系硫酸乙酰肝素（HS）修饰酶基因。代谢放射性标记的糖胺聚糖使我们能够评估果蝇糖胺聚糖的未表征的功能和HS结构和功能的突变的影响。新的体外系统将为我们提供果蝇HS的详细结构信息和在过去二十年中使用这种动物模型获得的生物表型数据的丰富知识之间的直接联系。
Genetic studies using a model organism, Drosophila melanogaster, have been contributing to elucidating the in vivo functions of heparan sulfate proteoglycans (HSPGs). On the other hand, biochemical analysis of Drosophila glycosaminoglycans (GAGs) has been limited, mainly due to the insufficient amount of the material obtained from the animal. Recently, a novel in vitro system has been developed by establishing mutant cell lines for heparan sulfate (HS)-modifying enzyme genes. Metabolic radiolabeling of GAGs allows us to assess uncharacterized features of Drosophila GAGs and the effects of the mutations on HS structures and function. The novel in vitro system will provide us with a direct link between detailed structural information of Drosophila HS and a wealth of knowledge on biological phenotypic data obtained over the last two decades using this animal model.