Phylogenetic diversity of microbial communities associated with the crude-oil, large-insoluble-particle and formation-water components of the reservoir fluid from a non-flooded high-temperature petroleum reservoir
Phylogenetic diversity of microbial communities associated with the crude-oil, large-insoluble-particle and formation-water components of the reservoir fluid from a non-flooded high-temperature petroleum reservoir
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DOI：
10.1016/j.jbiosc.2011.09.015
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发表时间：
2012-02-01
影响因子：
2.8
通讯作者：
Sato, Kozo
中科院分区：
文献类型：
--
作者：
Kobayashi, Hajime;Endo, Keita;Sato, Kozo
对新堀油田非水淹高温油藏微生物群落多样性进行了研究。饱和烃分析结果表明，该油藏原油中正构烷烃被选择性贫化，表明该油藏原油可能发生了轻度生物降解。为了检查是否有任何特定的微生物优先附着于原油或储层流体的其它组分（大的不溶性颗粒和地层水），从储层流体的每种组分构建16S rRNA基因克隆文库。古细菌文库中的克隆（共414个克隆）代表了16种类型，其中许多与产甲烷菌密切相关。细菌文库（共700个克隆）由属于16个门级分组之一的49个细菌型组成，厚壁菌门含有最大多样性的细菌型。在原油和大的不溶性颗粒相关的社区，甲烷菌相关的古菌序列占主导地位，而氢营养产甲烷菌占据了大部分的序列库中的地层水相关的社区。原油伴生菌群的多样性最高，共有35个菌型，其中16个菌型在其它菌群中未检出。因此，尽管与原油流体组分相关的种群在很大程度上共享共同的系统发育背景，但特定部分的微生物物种优先附着在原油和不溶性颗粒上。(C)2011年，生物技术学会，日本。All rights reserved.
The diversity of microbial communities associated with non-water-flooded high-temperature reservoir of the Niibori oilfield was characterized. Analysis of saturated hydrocarbons revealed that n-alkanes in crude oil from the reservoir were selectively depleted, suggesting that crude oil might be mildly biodegraded in the reservoir. To examine if any specific microorganism(s) preferentially attached to the crude oil or the other components (large insoluble particles and formation water) of the reservoir fluid, 16S rRNA gene clone libraries were constructed from each component of the reservoir fluid. The clones in the archaeal libraries (414 clones in total) represented 16 phylotypes, many of which were closely related to methanogens. The bacterial libraries (700 clones in total) were composed of 49 phylotypes belonging to one of 16 phylum-level groupings, with Firmicutes containing the greatest diversity of the phylotypes. In the crude-oil- and large-insoluble-particle-associated communities, a Methanosaeta-related phylotype dominated the archaeal sequences, whereas hydrogenotrophic methanogens occupied a major portion of sequences in the library of the formation-water-associated community. The crude-oil associated bacterial community showed the largest diversity, containing 35 phylotypes, 16 of which were not detected in the other bacterial communities. Thus, although the populations associated with the reservoir-fluid components largely shared common phylogenetic context, a specific fraction of microbial species preferentially attached to the crude oil and insoluble particles. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.