Induction of expression of cell-surface homologous restriction factor upon anti-CD3 stimulation of human peripheral lymphocytes.
Induction of expression of cell-surface homologous restriction factor upon anti-CD3 stimulation of human peripheral lymphocytes.
复制标题
人外周淋巴细胞抗 CD3 刺激后诱导细胞表面同源限制因子的表达。
DOI：
10.1073/pnas.85.1.213
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发表时间：
1988
影响因子：
11.1
通讯作者：
Müller-Eberhard,HJ
中科院分区：
文献类型：
--
作者：
Martin,DE;Zalman,LS;Müller-Eberhard,HJ
同源限制性内切因子（HRF）是一种65 kda的膜蛋白，可抑制补体的膜攻击复合体和补体成分c9相关的细胞溶解淋巴细胞蛋白的跨膜通道形成。用抗cd3单克隆抗体OKT3刺激静息的人外周血淋巴细胞已被证明可诱导CD8+亚群的细胞毒性。如本文所示，OKT3刺激也诱导CD4+和CD8+ T淋巴细胞表达细胞表面HRF。外周血单核细胞中少量Leu 19+自然杀伤淋巴细胞在刺激前表达HRF。未受刺激的外周血单个核细胞易受膜攻击复合物或c9相关蛋白的裂解，而受okt3刺激的外周血单个核细胞对膜攻击复合物和c9相关蛋白均具有相对抗性。用F（ab’）2抗HRF阻断表面HRF可消除这种获得性耐药，提示耐药是由于淋巴细胞膜HRF引起的。通过固相抗HRF，从活化的外周血单个核细胞中分离出65 kda蛋白，并证明其能够赋予绵羊红细胞与人HRF的特征活性。
Homologous restriction factor (HRF) is a 65-kDa membrane protein that inhibits transmembrane channel formation by the membrane-attack complex of complement and by the complement component C9-related cytolytic lymphocyte protein. Stimulation of resting peripheral human lymphocytes with the anti-CD3 monoclonal antibody OKT3 has been shown to induce cytotoxicity in the CD8+ subpopulation. As demonstrated here, OKT3 stimulation also induces expression of cell-surface HRF by CD4+ and CD8+ T lymphocytes. The small proportion of Leu 19+ natural killer lymphocytes present in peripheral blood mononuclear cells was found to express HRF prior to stimulation. Whereas unstimulated peripheral blood mononuclear cells were susceptible to lysis by the membrane-attack complex or by the C9-related protein, OKT3-stimulated peripheral blood mononuclear cells were relatively resistant to both the membrane-attack complex and C9-related protein. This acquired resistance was abrogated by blocking surface HRF with F(ab')2 anti-HRF, suggesting that resistance was due to lymphocyte-membrane HRF. By using solid-phase anti-HRF, a 65-kDa protein was isolated from the activated peripheral blood mononuclear cells and shown to be capable of conferring upon sheep erythrocytes the characteristic activity of human HRF.