Effective decellularisation of human saphenous veins for biocompatible arterial tissue engineering applications: Bench optimisation and feasibility in vivo testing.
Effective decellularisation of human saphenous veins for biocompatible arterial tissue engineering applications: Bench optimisation and feasibility in vivo testing.
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DOI：
10.1177/2041731420987529
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发表时间：
2021-01
影响因子：
8.2
通讯作者：
Ascione R
中科院分区：
文献类型：
--
作者：
Sulaiman NS;Bond AR;Bruno VD;Joseph J;Johnson JL;Suleiman MS;George SJ;Ascione R
人大隐静脉(HSV)和人造血管是血管移植中常用的管道，尽管失败率很高。脱细胞单纯疱疹病毒(D-HSV)制备血管支架可能是一种有效的替代方法。我们评估了使用0%至1%十二烷基硫酸钠(十二烷基硫酸钠)去细胞HSV的洗涤剂方法的有效性。通过核计数、DNA含量、剩余细胞活力、细胞外基质完整性和机械强度来评估去细胞效果。对人和猪细胞的细胞毒性进行了评估。最有效的十二烷基硫酸钠浓度被用于制备D-HSV移植物，该移植物使用猪颈动脉替代模型进行了初步的活体测试。0.01%的十二烷基硫酸钠可有效脱除细胞，且D-HSV接种后具有良好的生物相容性。体内异种移植证实了良好的机械强度和生物相容性，在没有机械故障的情况下重新招募宿主细胞，4周时通畅率为50%。我们已经开发了一种简单的生物兼容方法来有效地去除HSV的细胞。这可能会加强血管组织工程在未来的临床应用。
Human saphenous vein (hSV) and synthetic grafts are commonly used conduits in vascular grafting, despite high failure rates. Decellularising hSVs (D-hSVs) to produce vascular scaffolds might be an effective alternative. We assessed the effectiveness of a detergent-based method using 0% to 1% sodium dodecyl sulphate (SDS) to decellularise hSV. Decellularisation effectiveness was measured in vitro by nuclear counting, DNA content, residual cell viability, extracellular matrix integrity and mechanical strength. Cytotoxicity was assessed on human and porcine cells. The most effective SDS concentration was used to prepare D-hSV grafts that underwent preliminary in vivo testing using a porcine carotid artery replacement model. Effective decellularisation was achieved with 0.01% SDS, and D-hSVs were biocompatible after seeding. In vivo xeno-transplantation confirmed excellent mechanical strength and biocompatibility with recruitment of host cells without mechanical failure, and a 50% patency rate at 4-weeks. We have developed a simple biocompatible methodology to effectively decellularise hSVs. This could enhance vascular tissue engineering toward future clinical applications.
DOI：
10.5090/kjtcs.2013.46.3.165
发表时间：
2013-06
期刊：
The Korean journal of thoracic and cardiovascular surgery
影响因子：
--
作者：
Barner HB
通讯作者：
Barner HB