High glucose up-regulates angiotensin II subtype 2 receptors via interferon regulatory factor-1 in proximal tubule epithelial cells
High glucose up-regulates angiotensin II subtype 2 receptors via interferon regulatory factor-1 in proximal tubule epithelial cells
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DOI：
10.1007/s11010-010-0529-z
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发表时间：
2010-11-01
影响因子：
4.3
通讯作者：
Hussain, Tahir
中科院分区：
文献类型：
--
作者：
Ali, Quaisar;Sabuhi, Rifat;Hussain, Tahir
早期的研究报道了糖尿病患者近端小管AT（2）受体（AT（2）R）表达的增加，对肾功能和血压调节具有有益作用。在此，我们证明了AT（2）R蛋白表达的增加与高血糖大鼠和高糖处理的HK 2细胞中转录因子IRF-1表达的增加有关。在HK 2细胞中通过siRNA敲低IRF-1阻止高糖诱导的AT（2）R上调。这些数据表明，IRF-1是高血糖时AT（2）R表达的转录调节因子，需要进一步研究以了解IRF-1沿着AT（2）R在糖尿病肾脏中的生理作用。
Earlier studies have reported an increase in the proximal tubule AT(2) receptor (AT(2)R) expression in diabetes, with a beneficial role in kidney function and blood pressure regulation. Here, we demonstrate that the increase in AT(2)R protein expression is associated with an increased expression of transcription factor IRF-1 in hyperglycemic rat and in high glucose-treated HK2 cells. Knock-down of IRF-1 by siRNA in HK2 cells prevented high glucose-induced AT(2)R up-regulation. The data suggest that IRF-1 is a transcriptional regulator of AT(2)R expression in hyperglycemia, and warrant further studies to understand the physiological role of IRF-1 along with AT(2)R in diabetic kidney.