MiR-424-5p targets HSP90AA1 to facilitate proliferation and restrain differentiation in skeletal muscle development
MiR-424-5p targets HSP90AA1 to facilitate proliferation and restrain differentiation in skeletal muscle development
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DOI：
10.1080/10495398.2022.2102032
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发表时间：
2022-07-21
影响因子：
3.7
通讯作者：
Fang, Xingtang
中科院分区：
文献类型：
--
作者：
Chen, Xi;Zhu, Ying;Fang, Xingtang
MiR-424- 5 p被发现是多种癌细胞增殖、迁移和侵袭的潜在调节剂。然而，miR-424- 5 p在心肌发生过程中的作用及其机制尚不清楚。通过microRNA（miRNA）测序和表达分析，我们发现徐淮山羊骨骼肌中miR-424- 5 p的表达存在差异。我们推测miR-424- 5 p可能在骨骼肌肌发生中发挥重要作用。然后，我们发现小鼠成肌细胞（C2 C12成肌细胞系）的增殖能力显著增强，而增加miR-424- 5 p的表达后，C2 C12分化受到抑制。从机制上讲，HSP 90 AA 1与miR-424- 5 p存在密切的相互关系，miR-424- 5 p被预测为骨骼肌肌发生过程中的靶基因，使用转录组测序，双荧光素酶报告基因检测和qRT-PCR。HSP 90 AA 1的沉默明显增加C2 C12的增殖和减少分化，这与miR-424- 5 p在C2 C12中的能力一致。总之，我们的研究结果表明，miR-424- 5 p作为一种新的潜在的调节剂，通过HSP 90 AA 1在肌肉肌细胞发生过程中的作用。
MiR-424-5p was found to be a potential regulator in the proliferation, migration, and invasion of various cancer cells. However, the effects and functional mechanism of miR-424-5p in the process of myogenesis are still unclear. Previously, using microRNA (miRNA) sequencing and expression analysis, we discovered that miR-424-5p was expressed differentially in the different skeletal muscle growth periods of Xuhuai goats. We hypothesized that miR-424-5p might play an important role in skeletal muscle myogenesis. Then, we found that the proliferation ability of the mouse myoblast cell (C2C12 myoblast cell line) was significantly augmented, whereas the C2C12 differentiation was repressed after increasing the expression of miR-424-5p. Mechanistically, HSP90AA1 presented a close interrelation with miR-424-5p, which was predicted as a target gene in the progression of skeletal muscle myogenesis, using transcriptome sequencing, dual luciferase reporter gene detection, and qRT-PCR. The silencing of HSP90AA1 obviously increased C2C12 proliferation and diminished differentiation, which is consistent with the ability of miR-424-5p in C2C12. Altogether, our findings indicated the role of miR-424-5p as a novel potential regulator via HSP90AA1 during muscle myogenesis progression.