The effect of cyclic pressure on human monocyte-derived macrophages in vitro
The effect of cyclic pressure on human monocyte-derived macrophages in vitro
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DOI：
10.1302/0301-620x.82b5.9561
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发表时间：
2000-07-01
影响因子：
--
通讯作者：
Andrew, JG
中科院分区：
文献类型：
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作者：
Ferrier, GM;McEvoy, A;Andrew, JG
假体关节周围的无菌性松动和骨溶解是失败和随后翻修的主要原因。在这个过程中，激活的巨噬细胞产生细胞因子，这种细胞因子被认为可以促进破骨细胞的骨溶解。种植体周围间隙压力的变化被认为是松动和骨溶解的原因，因此我们研究了两种不同的循环压力对培养的人单核细胞来源的巨噬细胞产生IL-1β、IL-6和肿瘤坏死因子-α的影响。不同供体的未刺激的M-D巨噬细胞的细胞因子表达存在很大差异，因此将同一供体的细胞在对照和加压条件下进行比较。IL-1β的表达仅在较高频率的方案中增加。我们的发现表明，M-D巨噬细胞在循环压力下被激活。需要进一步的工作来理解施加压力的频率、幅度和持续时间在该系统中循环压力的细胞效应中的相对作用。
Aseptic loosening and osteolysis around prosthetic joints are the principal causes of failure and consequent revision. During this process activated macrophages produce cytokines which are thought to promote osteolysis by osteoclasts. Changes in pressure within the space around implants have been proposed as a cause of loosening and osteolysis, We therefore studied the effect of two different regimes of cyclic pressure on the production of interleukin-1 beta (IL-1 beta), IL-6 and tumour necrosis factor-alpha (TNF-alpha) by cultured human monocyte-derived (M-D) macrophages, There was a wide variation in the expression of cytokines in non-stimulated M-D macrophages from different donors and therefore cells from the same donor were compared under control and pressurised conditions.Both regimes of cyclic pressure were found to increase expression of IL-6 and TNF-alpha. Expression of IL-1 beta was increased by a higher-frequency regime only. Our findings suggest that M-D macrophages are activated by cyclic pressure. Further work will be required to understand the relative roles of frequency, amplitude and duration of applied pressure in the cellular effects of cyclic pressure in this system.