Triglycidyl amine crosslinking combined with ethanol inhibits bioprosthetic heart valve calcification.
Triglycidyl amine crosslinking combined with ethanol inhibits bioprosthetic heart valve calcification.
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DOI：
10.1016/j.athoracsur.2011.04.104
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发表时间：
2011-09
影响因子：
4.6
通讯作者：
Levy, Robert J.
中科院分区：
文献类型：
--
作者：
Connolly, Jeanne M.;Bakay, Marina A.;Alferiev, Ivan S.;Gorman, Robert C.;Gorman, Joseph H., III;Kruth, Howard S.;Ashworth, Paul E.;Kutty, Jaishankar K.;Schoen, Frederick J.;Bianco, Richard W.;Levy, Robert J.
导致生物人工心脏瓣膜钙化衰竭的最重要因素之一是戊二醛交联。此前曾有报道称，戊二醛交联后进行乙醇 (EtOH) 孵育可赋予生物假体抗钙化功效。目前的研究调查了新型交联剂三缩水甘油胺 (TGA) 在有或没有 EtOH 孵育的情况下与戊二醛相比的体内抗钙化功效。 TGA 交联 (+/- EtOH) 用于制备用于大鼠皮下植入物和绵羊二尖瓣置换的猪主动脉瓣，以便与戊二醛固定对照进行比较。对热变性温度（Ts）、交联指数、胆固醇提取和流体动力学特性进行了量化。外植体终点包括钙化的定量和形态学评估。 TGA 后的 Ts 介于未固定和戊二醛固定之间。 EtOH 孵育导致从 TGA 或戊二醛固定尖点中几乎完全提取胆固醇。大鼠皮下外植体（90 天）表明，与其他条件相比，TGA-EtOH 对钙化的抑制作用显着更高。因此，制造了安装有 TGA-乙醇支架的猪主动脉瓣生物假体，用于与戊二醛预处理的对照进行比较。在流体动力学研究中，TGA-EtOH 生物假体的压力梯度比戊二醛固定的低。与戊二醛固定对照相比，TGA-乙醇生物假体在幼年羊（150 天）中用作二尖瓣替代物，显示出移植猪主动脉瓣和主动脉壁样本中的钙水平显着降低。然而，TGA-EtOH 绵羊外植体也显示出孤立的钙化结节和牙尖内血肿。 TGA-EtOH 预处理猪主动脉瓣可在大鼠皮下和绵羊循环植入物中赋予显着的钙化抗性，但伴随着结构不稳定。
One of the most important factors responsible for the calcific failure of bioprosthetic heart valves is glutaraldehyde cross-linking. Ethanol (EtOH) incubation after glutaraldehyde cross-linking has previously been reported to confer anti-calcification efficacy for bioprostheses. The present studies investigated the anticalcification efficacy in vivo of the novel cross-linking agent, triglycidyl amine (TGA), with or without EtOH incubation, in comparison to glutaraldehyde. TGA cross-linking (+/− EtOH) was used to prepare porcine aortic valves for both rat subdermal implants and sheep mitral valve replacements, for comparisons with glutaraldehyde-fixed controls. Thermal denaturation temperature (Ts), an index of cross-linking, cholesterol extraction, and hydrodynamic properties were quantified. Explant endpoints included quantitative and morphologic assessment of calcification. Ts after TGA were intermediate between unfixed and glutaraldehyde-fixed. EtOH incubation resulted in almost complete extraction of cholesterol from TGA or glutaraldehyde-fixed cusps. Rat subdermal explants (90days) demonstrated that TGA-EtOH resulted in a significantly greater level of inhibition of calcification than other conditions. Thus, TGA-ethanol stent mounted porcine aortic valve bioprostheses were fabricated for comparisons with glutaraldehyde-pretreated controls. In hydrodynamic studies, TGA-EtOH bioprostheses had lower pressure gradients than glutaraldehyde-fixed. TGA-ethanol bioprostheses used as mitral valve replacements in juvenile sheep (150 days) demonstrated significantly lower calcium levels in both explanted porcine aortic cusp and aortic wall samples compared to glutaraldehyde-fixed controls. However, TGA-EtOH sheep explants also demonstrated isolated calcific nodules and intracuspal hematomas. TGA-EtOH pretreatment of porcine aortic valves confers significant calcification resistance in both rat subdermal and sheep circulatory implants, but with associated structural instability.
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影响因子：
14
作者：
Rapoport, H. Scott;Connolly, Jeanne M.;Levy, Robert J.
通讯作者：
Levy, Robert J.
影响因子：
3.7
作者：
Schoen, FJ
通讯作者：
Schoen, FJ
DOI：
10.1002/(sici)1097-4636(19980615)40:4
发表时间：
1998-06-15
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Vyavahare, NR;Hirsch, D;Levy, RJ
通讯作者：
Levy, RJ
影响因子：
2.3
作者：
Alferiev, IS;Connolly, JM;Levy, RJ
通讯作者：
Levy, RJ
影响因子：
2.8
作者：
SANDERS, SP;LEVY, RJ;CASTANEDA, AR
通讯作者：
CASTANEDA, AR