New fluorochromes, compatible with high wavelength excitation, for flow cytometric analysis of cellular nucleic acids.
New fluorochromes, compatible with high wavelength excitation, for flow cytometric analysis of cellular nucleic acids.
复制标题
新型荧光染料，与高波长激发兼容，用于细胞核酸的流式细胞术分析。
DOI：
10.1002/cyto.990050409
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Lalande,M
中科院分区：
文献类型：
--
作者：
Latt,SA;Marino,M;Lalande,M
The spectroscopic properties of three new dyes, EK4, VL772, and LL585, free and bound to nucleic acids, are presented, with particular emphasis on their potential use in flow cytometry. Two of these dyes, EK4 and LL585, exhibit red fluorescence, while dye VL772 exhibits yellow fluorescence. Dye LL585 exhibits specificity for DNA, relative to RNA, and a marked enhancement of fluorescence efficiency upon binding to DNA, needed for a red fluorescent DNA‐specific stain for flow cytometry. The dye penetrates live cells, although uniformity of nuclear fluorescence, as evidenced by DNA flow histograms, is better if the cells are first permeabilized with Triton X‐100. Dye VL772 exhibits yellow fluorescence and little DNA‐RNA discrimination, but may prove useful in conjunction with dye LL585 when simultaneous assay of cellular RNA and DNA is desired. Dye EK4 shares properties of the other two dyes but fluoresces with much less efficiency. Dyes LL585 and VL772, used singly, as a pair, or in combination with blue‐fluorescing DNA specific dyes, such as bisbenzimidazole derivatives, should permit new, convenient analyses of the content and organization of cellular nucleic acids.
