Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
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DOI：
10.1371/journal.pone.0053543
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发表时间：
2013-01-11
期刊：
影响因子：
3.7
通讯作者：
Pinto, Gloria
中科院分区：
文献类型：
--
作者：
Correia, Barbara;Valledor, Luis;Pinto, Gloria
树木必然会经历温度的变化，需要有效的短期策略，这对环境变化的适应性至关重要。DNA甲基化和组蛋白翻译后修饰通过控制染色质的功能状态和基因表达，在表观遗传控制和胁迫下的植物功能状态中起着关键作用。软木栎（Quercus suber L.）是地中海地区的关键石，生长在45摄氏度的温度下。该物种在实验室条件下经历了从25摄氏度到55摄氏度的累积温度升高，以测试表观遗传密码与热应激耐受性相关的假设。电解质渗漏在35 ℃后增加，但所有植物都能存活到55 ℃。通过HPCE（高效毛细管电泳）、MS-RAPD（甲基化敏感随机扩增多态性DNA）和蛋白质凝胶印迹分析监测DNA甲基化和乙酰化组蛋白H3（AcH 3）水平，并使用共聚焦显微镜评估修饰的空间分布。HPCE分析的DNA甲基化显示在55 ℃时有所增加，而MS-RAPD结果表明在压力下动态甲基化-去甲基化模式。蛋白质凝胶印迹显示AcH 3的丰度指数从25 ℃降低到45 ℃。5-mC（5-methyl-2 '-deoxycytidine）和AcH_3的免疫组化检测与上述结果一致。这些结果表明，表观遗传机制，如DNA甲基化和组蛋白H3乙酰化具有相反的和特殊的动力学，这可能是至关重要的逐步建立这种物种进入这样的高应力（55摄氏度），使其适应和生存。这是第一份评估表观遗传调控以研究林木耐热性的报告。
Trees necessarily experience changes in temperature, requiring efficient short-term strategies that become crucial in environmental change adaptability. DNA methylation and histone posttranslational modifications have been shown to play a key role in both epigenetic control and plant functional status under stress by controlling the functional state of chromatin and gene expression. Cork oak (Quercus suber L.) is a key stone of the Mediterranean region, growing at temperatures of 45 degrees C. This species was subjected to a cumulative temperature increase from 25 degrees C to 55 degrees C under laboratory conditions in order to test the hypothesis that epigenetic code is related to heat stress tolerance. Electrolyte leakage increased after 35 degrees C, but all plants survived to 55 degrees C. DNA methylation and acetylated histone H3 (AcH3) levels were monitored by HPCE (high performance capillary electrophoresis), MS-RAPD (methylation-sensitive random-amplified polymorphic DNA) and Protein Gel Blot analysis and the spatial distribution of the modifications was assessed using a confocal microscope. DNA methylation analysed by HPCE revealed an increase at 55 degrees C, while MS-RAPD results pointed to dynamic methylation-demethylation patterns over stress. Protein Gel Blot showed the abundance index of AcH3 decreasing from 25 degrees C to 45 degrees C. The immunohistochemical detection of 5-mC (5-methyl-2'-deoxycytidine) and AcH3 came upon the previous results. These results indicate that epigenetic mechanisms such as DNA methylation and histone H3 acetylation have opposite and particular dynamics that can be crucial for the stepwise establishment of this species into such high stress (55 degrees C), allowing its acclimation and survival. This is the first report that assesses epigenetic regulation in order to investigate heat tolerance in forest trees.