PRELI (protein of relevant evolutionary and lymphoid interest) is located within an evolutionarily conserved gene cluster on chromosome 5q34-q35 and encodes a novel mitochondrial protein
PRELI (protein of relevant evolutionary and lymphoid interest) is located within an evolutionarily conserved gene cluster on chromosome 5q34-q35 and encodes a novel mitochondrial protein
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DOI：
10.1042/bj20031504
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发表时间：
2004-03-15
影响因子：
4.1
通讯作者：
Wright, SC
中科院分区：
文献类型：
--
作者：
Fox, EJ;Stubbs, SA;Wright, SC
线粒体蛋白的表征对于理解正常细胞功能和线粒体疾病都很重要。在本研究中，我们鉴定了一种新的线粒体蛋白PRELI（相关进化和淋巴感兴趣的蛋白），该蛋白编码在位于染色体5q34-q35的进化保守的MAD3/PRELI/RAB24基因簇中。小鼠Preli在分析的所有环境中都有高水平表达；它通过一个强双向启动子与Rab24共表达，并且独立于位于其3'端的s期特异性Mad3基因进行调控。PRELI在其n端包含一个独立的170个氨基酸的PRELI/MSF1p'基序。该结构域存在于酵母、果蝇和哺乳动物等真核生物的多种蛋白质中，但其功能尚不清楚，而高等真核生物PRELI/MSFIP蛋白的亚细胞位置此前尚未确定。我们在这里表明PRELI位于线粒体中，通过使用绿色荧光蛋白融合蛋白，我们在其n端识别了线粒体靶向信号。
The characterization of mitochondrial proteins is important for the understanding of both normal cellular function and mitochondrial disease. In the present study we identify a novel mitochondrial protein, PRELI (protein of relevant evolutionary and lymphoid interest), that is encoded within the evolutionarily conserved MAD3/PRELI/RAB24 gene cluster located at chromosome 5q34-q35. Mouse Preli is expressed at high levels in all settings analysed; it is co-expressed with Rab24 from a strong bi-directional promoter, and is regulated independently from the S-phase-specific Mad3 gene located at its 3' end. PRELI contains a stand-alone 170 amino acid PRELI/MSF1p' motif at its N-terminus. This domain is found in a variety of proteins from diverse eukaryotes including yeast, Drosophila and mammals, but its function is unknown, and the subcellular location of higher eukaryotic PRELI/MSFIP' proteins has not been determined previously. We show here that PRELI is located in the mitochondria, and by using green-fluorescent-protein fusion proteins we identify a mitochondrial targeting signal at its N-terminus.