Excitable systems: A new perspective on the cellular impact of elongate mineral particles
Excitable systems: A new perspective on the cellular impact of elongate mineral particles
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DOI：
10.1016/j.envres.2023.115353
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发表时间：
2023-05-26
影响因子：
8.3
通讯作者：
Losert，Wolfgang
中科院分区：
文献类型：
--
作者：
Gu，Shuyao;Bull，Abby;Losert，Wolfgang
我们研究了细长矿物颗粒（EMP）的几何形状与细胞接触的影响，最近发现的纹理传感机制的esotaxis。趋异性是基于细胞骨架波和振荡，这些波和振荡由周围的纹理成核、成形和操纵。我们发现，所有研究的电磁脉冲触发巨噬细胞的内向反应，这种反应主导这些免疫细胞的细胞骨架活性。相比之下，上皮细胞对EMP几乎没有或没有外趋反应。这些结果与两种细胞类型的不同相互作用一致，其尺寸与石棉状EMP的尺寸相当。我们的研究结果提出了一个问题，即狭窄的石棉状电磁脉冲是否也可能主导其他类型的免疫细胞，表现出类似的外趋化作用的细胞骨架活性。这些发现，加上先前的研究的esotaxis，导致我们的假设，石棉状电磁脉冲抑制免疫细胞的迁移和激活免疫信号，从而竞争的信号，通常会刺激免疫系统在附近的组织。
We investigate how the geometry of elongate mineral particles (EMPs) in contact with cells influences esotaxis, a recently discovered mechanism of texture sensing. Esotaxis is based on cytoskeletal waves and oscillations that are nucleated, shaped, and steered by the texture of the surroundings. We find that all EMPs studied trigger an esotactic response in macrophages, and that this response dominates cytoskeletal activity in these immune cells. In contrast, epithelial cells show little to no esotactic response to the EMPs. These results are consistent with the distinct interactions of both cell types with ridged nanotopographies of dimensions comparable to those of asbestiform EMPs. Our findings raise the question of whether narrow, asbestiform EMPs may also dominate cytoskeletal activity in other types of immune cells that exhibit similar esotactic effects. These findings, together with prior studies of esotaxis, lead us to the hypothesis that asbestiform EMPs suppress the migration of immune cells and activate immune signaling, thereby outcompeting signals that would normally stimulate the immune system in nearby tissue.